Rat Macrophage Migration Inhibitory Factor, MIF ELISA Kit | MyBioSource MBS702992 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat Macrophage Migration Inhibitory Factor, MIF ELISA Kit

catalog :

MBS702992

quantity :

48-Strip-Wells

price :

465 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS702992

products type :

ELISA Kit

products full name :

Rat Macrophage Migration Inhibitory Factor, MIF ELISA Kit

products short name :

macrophage migration inhibitory factor (glycosylation-inhibiting factor)

products name syn :

Rat Macrophage Migration Inhibitory Factor; MIF ELISA Kit; GIF; GLIF; MMIF; macrophage migration inhibitory factor; phenylpyruvate tautomerase; macrophage migration inhibitory factor (glycosylation-inhibiting factor)

other names :

macrophage migration inhibitory factor; Macrophage migration inhibitory factor; macrophage migration inhibitory factor; L-dopachrome isomerase; L-dopachrome tautomerase; phenylpyruvate tautomerase; glutathione-binding 13 kDa protein; macrophage migration inhibitory factor (glycosylation-inhibiting factor); Glutathione-binding 13 kDa protein; L-dopachrome isomerase; L-dopachrome tautomerase (EC:5.3.3.12); Phenylpyruvate tautomerase

products gene name :

MIF

other gene names :

Mif; Mif; MIF

uniprot entry name :

MIF_RAT

reactivity :

Rat

sequence length :

115

specificity :

This assay has high sensitivity and excellent specificity for detection of rat MIF. No significant cross-reactivity or interference between rat MIF and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Sandwich. Detection Range: 62.5 pg/ml -4000 pg/ml. Sensitivity: The minimum detectable dose of rat MIF is typically less than 15.6 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MIF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MIF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MIF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MIF bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

13591985

ncbi acc num :

NP_112313.1

ncbi gb acc num :

NM_031051.1

uniprot acc num :

P30904

ncbi mol weight :

12,477 Da

ncbi pathways :

Adipogenesis Pathway (198479); Phenylalanine Metabolism Pathway (83352); Phenylalanine Metabolism Pathway (327); Tyrosine Metabolism Pathway (83351); Tyrosine Metabolism Pathway (325)

ncbi summary :

inhibits random migration of macrophages and is involved in the pathogenesis of several inflammatory diseases [RGD, Feb 2006]

uniprot summary :

Function: Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity . Catalytic activity: Keto-phenylpyruvate = enol-phenylpyruvate.

size1 :

48-Strip-Wells

price1 :

465 USD

size2 :

96-Strip-Wells

price2 :

655

size3 :

5x96-Strip-Wells

price3 :

2455

size4 :

10x96-Strip-Wells

price4 :

4595

more info or order :

MyBioSource product webpage