catalog number :
MBS702966
products type :
ELISA Kit
products full name :
Mouse Thyroid-Peroxidase, TPO ELISA Kit
products short name :
thyroid peroxidase
products name syn :
Mouse Thyroid-Peroxidase; TPO ELISA Kit; MSA; TDH2A; TPX; OTTHUMP00000115536; thyroid microsomal antigen; thyroperoxidase; thyroid peroxidase
other names :
thyroid peroxidase; Thyroid peroxidase; thyroid peroxidase; thyroid peroxidase
other gene names :
Tpo; Tpo; TPO
uniprot entry name :
PERT_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of Mouse TPO. No significant cross-reactivity or interference between Mouse TPO and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Assay Type: Competitive
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Oxidative Stress. Protein Biological Process 3: Hydrogen peroxide
products description :
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to TPO has been pre-coated onto a microplate. Standards or samples are added to the appropriate microtiter plate wells with biotin-conjugated TPO and incubated. A competitive inhibition reaction is launched between TPO (Standards or samples) and Biotin-conjugated TPO with the pre-coated antibody specific for TPO. The more amount of TPO in samples, the less antibody bound by Biotin-conjugated TPO. Then Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The substrate solutions are added to the wells, respectively. And the color develops in opposite to the amount of TPO in the sample. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_033443.1
ncbi gb acc num :
NM_009417.2
ncbi mol weight :
101,342 Da
ncbi pathways :
Amine-derived Hormones Pathway (1001139); Amino Acid Metabolism Pathway (198416); Autoimmune Thyroid Disease Pathway (83314); Autoimmune Thyroid Disease Pathway (533); Cytokine-cytokine Receptor Interaction Pathway (83248); Cytokine-cytokine Receptor Interaction Pathway (460); Hematopoietic Cell Lineage Pathway (83275); Hematopoietic Cell Lineage Pathway (489); Jak-STAT Signaling Pathway (83274); Jak-STAT Signaling Pathway (488)
uniprot summary :
Function: Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T3 and T4. Catalytic activity: 2 iodide + H2O2 + 2 H+ = 2 iodine + 2 H2O. Cofactor: Binds 1 calcium ion per heterodimer
size4 :
10x96-Strip-Wells