product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Human pigment epithelium-derived factor, PEDF ELISA Kit
catalog :
MBS702956
quantity :
48-Strip-Wells
price :
510 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS702956
products type :
ELISA Kit
products full name :
Human pigment epithelium-derived factor, PEDF ELISA Kit
products short name :
pigment epithelium-derived factor, PEDF
products name syn :
Human pigment epithelium-derived factor; PEDF ELISA Kit; EPC-1; PEDF; PIG35; pigment epithelium-derived factor; proliferation-inducing protein 35; serine (or cysteine) proteinase inhibitor; clade F (alpha-2 antiplasmin; pigment epithelium derived factor) ; pigment epithelium-derived factor; PEDF
other names :
pigment epithelium-derived factor; Pigment epithelium-derived factor; pigment epithelium-derived factor; serpin F1; cell proliferation-inducing gene 35 protein; serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1; serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1; Cell proliferation-inducing gene 35 protein; EPC-1; Serpin F1
products gene name :
PEDF
other gene names :
SERPINF1; SERPINF1; OI6; OI12; PEDF; EPC-1; PIG35; PEDF; PIG35; PEDF
uniprot entry name :
PEDF_HUMAN
reactivity :
Human
sequence length :
418
specificity :
This assay has high sensitivity and excellent specificity for detection of human PEDF. No significant cross-reactivity or interference between human PEDF and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, urine, cell culture supernates, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.156 ng/ml -10 ng/ml. Sensitivity: 0.039 ng/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PEDF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PEDF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PEDF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PEDF bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi gi num :
39725934
ncbi acc num :
NP_002606.3
ncbi gb acc num :
NM_002615.5
uniprot acc num :
P36955
ncbi mol weight :
46,312 Da
ncbi summary :
The protein encoded by this gene is a member of the serpin family, although it does not display the serine protease inhibitory activity shown by many of the other serpin family members. The encoded protein is secreted and strongly inhibits angiogenesis. In addition, this protein is a neurotrophic factor involved in neuronal differentiation in retinoblastoma cells.[provided by RefSeq, Mar 2011]
uniprot summary :
Function: Neurotrophic protein; induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.2 PublicationsManual assertion based on experiment in:Ref.13
size1 :
48-Strip-Wells
price1 :
510 USD
size2 :
96-Strip-Wells
price2 :
725
size3 :
5x96-Strip-Wells
price3 :
2565
size4 :
10x96-Strip-Wells
price4 :
4800
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

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Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

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