ELISA/assay

Mouse Soluble Toll-like Receptor 9, sTLR9/sCD289 ELISA Kit

MBS702953

48-Strip-Wells

590 USD

ELISA Kit

Mouse Soluble Toll-like Receptor 9, sTLR9/sCD289 ELISA Kit

toll-like receptor 9

Mouse Soluble Toll-like Receptor 9; sTLR9/sCD289 ELISA Kit; CD289; ; toll-like receptor 9

toll-like receptor 9; Toll-like receptor 9; toll-like receptor 9; toll-like receptor 9; CD_antigen: CD289

TLR9

Tlr9; Tlr9

TLR9_MOUSE

Mouse

1032

This assay has high sensitivity and excellent specificity for detection of Mouse TLR9. No significant cross-reactivity or interference between Mouse TLR9 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Assay Type: Sandwich

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Immunity. Protein Biological Process 3: Immunity

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for sTLR9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any sTLR9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for sTLR9 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of sTLR9 bound in the initial step. The color development is stopped and the intensity of the color is measured.

157057166

NP_112455.2

NM_031178.2

Q9EQU3

116,412 Da

African Trypanosomiasis Pathway (194387); African Trypanosomiasis Pathway (194323); Chagas Disease (American Trypanosomiasis) Pathway (147810); Chagas Disease (American Trypanosomiasis) Pathway (147795); Herpes Simplex Infection Pathway (377874); Herpes Simplex Infection Pathway (377865); IGF1R Signaling Cascade Pathway (971219); IRS-mediated Signalling Pathway (971221); IRS-related Events Pathway (970874); IRS-related Events Triggered By IGF1R Pathway (971223)

Function: Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Plays a role in defense against systemic mouse cytomegalovirus infection. Controls lymphocyte response to Helicobacter infection.2 PublicationsManual assertion based on experiment in:Ref.5

48-Strip-Wells

590 USD

96-Strip-Wells

850

5x96-Strip-Wells

2880

10x96-Strip-Wells

5440