catalog number :
MBS702943
products type :
ELISA Kit
products full name :
Human Protamine 1, PRM1 ELISA Kit
products short name :
protamine 1
products name syn :
Human Protamine 1; PRM1 ELISA Kit; CT94.1; P1; cancer/testis antigen family 94; member 1; sperm protamine P1; testis specific protamine 1; protamine 1
other names :
sperm protamine P1; Sperm protamine P1; sperm protamine P1; cysteine-rich protamine; testis specific protamine 1; cancer/testis antigen family 94, member 1; protamine 1; Cysteine-rich protamine
products gene name :
PRM1
other gene names :
PRM1; PRM1; P1; CT94.1
uniprot entry name :
HSP1_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of human PRM1. No significant cross-reactivity or interference between human PRM1 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma. Assay Type: Sandwich. Detection Range: 31.25 pg/ml -2000 pg/ml. Sensitivity: 7.8 pg/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PRM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PRM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRM1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRM1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_002752.1
ncbi gb acc num :
NM_002761.2
ncbi mol weight :
6,823 Da
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)
uniprot summary :
Function: Protamines substitute for histones in the chromatin of sperm during the haploid phase of spermatogenesis. They compact sperm DNA into a highly condensed, stable and inactive complex.
size4 :
10x96-Strip-Wells