catalog number :
MBS702937
products type :
ELISA Kit
products full name :
Mouse basic fibroblast growth factor, bFGF ELISA Kit
products short name :
fibroblast growth factor 2 (basic)
products name syn :
Mouse basic fibroblast growth factor; bFGF ELISA Kit; BFGF; FGFB; HBGF-2; basic fibroblast growth factor bFGF; fibroblast growth factor 2; heparin-binding growth factor 2; prostatropin; fibroblast growth factor 2 (basic)
other names :
fibroblast growth factor 2; Fibroblast growth factor 2; fibroblast growth factor 2; HBGF-2; basic fibroblast growth factor; heparin-binding growth factor 2; fibroblast growth factor 2; Basic fibroblast growth factor; bFGF; Heparin-binding growth factor 2; HBGF-2
products gene name :
FGF2
other gene names :
Fgf2; Fgf2; Fgfb; bFGF; Fgf-2; Fgf-2; FGF-2; bFGF; HBGF-2
uniprot entry name :
FGF2_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse bFGF. No significant cross-reactivity or interference between mouse bFGF and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Sandwich. Detection Range: 3.12 pg/ml-200 pg/ml. Sensitivity: Typically less than 0.78 pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for bFGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any bFGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for bFGF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of bFGF bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_032032.1
ncbi gb acc num :
NM_008006.2
ncbi mol weight :
17,153 Da
ncbi pathways :
Activated Point Mutants Of FGFR2 Pathway (1000637); Adaptive Immune System Pathway (971210); Constitutive PI3K/AKT Signaling In Cancer Pathway (1000676); DAP12 Interactions Pathway (971203); DAP12 Signaling Pathway (971214); Developmental Biology Pathway (970813); Disease Pathway (970606); Downstream Signal Transduction Pathway (971469); Downstream Signaling Events Of B Cell Receptor (BCR) Pathway (1001193); Downstream Signaling Of Activated FGFR Pathway (971322)
uniprot summary :
Function: Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro
size4 :
10x96-Strip-Wells