catalog number :
MBS702896
products type :
ELISA Kit
products full name :
Rat Leptin Soluble Receptor, sLR ELISA Kit
products short name :
[leptin receptor]
products name syn :
[Rat Leptin Soluble Receptor; sLR ELISA Kit; CD295; OBR; OB receptor; OTTHUMP00000010694; leptin receptor]
other names :
[leptin receptor; Leptin receptor; leptin receptor; OB-R; LEP-R; OB receptor; Leptin receptor (fatty); leptin receptor; OB receptor; OB-R; CD_antigen: CD295]
products gene name :
[LEPR]
other gene names :
[Lepr; Lepr; Fa; Fa; Obr; LEP-R; OB-R]
uniprot entry name :
LEPR_RAT
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse sLOX-1. No significant cross-reactivity or interference between mouse sLOX-1 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Tissue Homogenates. Assay Type: Quantitative Sandwich. Detection Range: 78 pg/ml-5000 pg/ml. Sensitivity: 19.5 pg/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for sLOX-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any sLOX-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for sLOX-1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of sLOX-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_036728.1
ncbi gb acc num :
NM_012596.1
ncbi mol weight :
130,833 Da
ncbi pathways :
AMPK Signaling Pathway (989832); AMPK Signaling Pathway (992181); Adipocytokine Signaling Pathway (83485); Adipocytokine Signaling Pathway (505); Cytokine-cytokine Receptor Interaction Pathway (83443); Cytokine-cytokine Receptor Interaction Pathway (460); Jak-STAT Signaling Pathway (83469); Jak-STAT Signaling Pathway (488); Neuroactive Ligand-receptor Interaction Pathway (83445); Neuroactive Ligand-receptor Interaction Pathway (462)
ncbi summary :
receptor for leptin; may have a role in body weight maintainence [RGD, Feb 2006]
uniprot summary :
Function: Receptor for obesity factor (leptin). On ligand binding, mediates signaling through JAK2/STAT3 Involved in the regulation of fat metabolism and, in a hematopoietic pathway, required for normal lymphopoiesis. May play a role in reproduction. Can also mediate the ERK/FOS signaling pathway
size1 :
24-Strip-Wells (LIMIT 1)
size5 :
10x96-Strip-Wells