ELISA/assay

Human alpha-amylase, AMY1 ELISA Kit

MBS702886

48-Strip-Wells

565 USD

ELISA Kit

Human alpha-amylase, AMY1 ELISA Kit

alpha-amylase

Human alpha-amylase (AMY1) ELISA Kit; 1; 4-alpha-D-glucan glucanohydrolase 1; Salivary alpha-amylase; AMY1A; AMY1B; AMY1C; alpha-amylase

alpha-amylase 1; Alpha-amylase 1; alpha-amylase 1; glycogenase; salivary alpha-amylase; salivary amylase alpha 1B; amylase, salivary, alpha-1B; 1,4-alpha-D-glucan glucanohydrolase 1; amylase, alpha 1B (salivary); 1,4-alpha-D-glucan glucanohydrolase 1; Salivary alpha-amylase

AMY1

AMY1B; AMY1A; AMY1; AMY1 AMY1B ; AMY1 AMY1C ; AMY1

AMY1_HUMAN

Human

511

This assay has high sensitivity and excellent specificity for detection of human AMY1. No significant cross-reactivity or interference between human AMY1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, saliva, tissue homogenates. Assay Type: Sandwich. Detection Range: 7.8 IU/ml-500 IU/ml. Sensitivity: The minimum detectable dose of human AMY1 is typically less than 1.95 IU/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for AMY1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AMY1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AMY1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AMY1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

56549662

NP_001008219.1

NM_001008218.1

P04745

57,768 Da

Carbohydrate Digestion And Absorption Pathway (170720); Carbohydrate Digestion And Absorption Pathway (170654); Digestion Of Dietary Carbohydrate Pathway (106197); Disease Pathway (530764); Glycogen Storage Diseases Pathway (980468); Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Carbohydrates Pathway (106196); Myoclonic Epilepsy Of Lafora Pathway (980469); Salivary Secretion Pathway (153376)

Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the salivary gland. [provided by RefSeq, Jul 2008]

AMY1: Monomer. Belongs to the glycosyl hydrolase 13 family. Protein type: Secreted, signal peptide; EC 3.2.1.1; Secreted; Carbohydrate Metabolism - starch and sucrose; Hydrolase. Chromosomal Location of Human Ortholog: 1p21. Cellular Component: extracellular space. Molecular Function: alpha-amylase activity; protein binding; metal ion binding. Biological Process: digestion; carbohydrate metabolic process

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150