ELISA/assay

Bovine transforming growth factor beta1, TGF-beta1 ELISA Kit

MBS702883

96 Strip Wells

850 USD

ELISA Kit

Bovine transforming growth factor beta1, TGF-beta1 ELISA Kit

transforming growth factor, beta 1

Bovine transforming growth factor beta1; TGF-beta1 ELISA Kit; CED; DPD1; LAP; TGFB; TGFbeta; TGF-beta 1 protein; latency-associated peptide; transforming growth factor; beta 1

transforming growth factor beta-1; Transforming growth factor beta-1; transforming growth factor beta-1; TGF-beta-1; transforming growth factor-beta 1; transforming growth factor, beta 1 (Camurati-Engelmann disease)

TGFB1

TGFB1; TGFB1; TGF-beta-1; LAP

TGFB1_BOVIN

Bovine

390

This assay has high sensitivity and excellent specificity for detection of Bovine TGFB1. No significant cross-reactivity or interference between Bovine TGFB1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, cell culture supernates. Detection Range: 6.25 ng/ml-400 ng/ml. Detection Wavelength: 450 nm. Sensitivity: 1.56 ng/ml

Sample Volume: 50-100ul

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to TGF-beta1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for TGF-beta1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain TGF-beta1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of TGF-beta1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

260655603

NP_001159540.1

NM_001166068.1

P18341

44,409 Da

Adipogenesis Pathway 920913!!Amoebiasis Pathway 167315!!Amoebiasis Pathway 167191!!Cell Cycle Pathway 920945!!Cell Cycle Pathway 84293!!Cell Cycle Pathway 463!!Chagas Disease (American Trypanosomiasis) Pathway 147808!!Chagas Disease (American Trypanosomiasis) Pathway 147795!!Chronic Myeloid Leukemia Pathway 84351!!Chronic Myeloid Leukemia Pathway 528

Function: Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts

96 Strip Wells

850 USD