ELISA/assay

Rat vimentin, VIM ELISA Kit

MBS702882

24-Strip-Wells (LIMIT 1)

240 USD

ELISA Kit

Rat vimentin, VIM ELISA Kit

[vimentin]

[Rat vimentin (VIM) ELISA Kit; RP11-124N14.1; FLJ36605; ; vimentin]

[vimentin; Vimentin; vimentin; vimentin]

[VIM]

[Vim; Vim]

VIME_RAT

Rat

466

This assay has high sensitivity and excellent specificity for detection of human VIM. No significant cross-reactivity or interference between human VIM and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, Plasma, Tissue Homogenates. Assay Type: Quantitative Sandwich. Detection Range: 31.25 pg/ml-2000 pg/ml. Sensitivity: 7.8 pg/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for VIM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any VIM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for VIM is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of VIM bound in the initial step. The color development is stopped and the intensity of the color is measured.

14389299

NP_112402.1

NM_031140.1

P31000

53,733 Da

Alpha6-Beta4 Integrin Signaling Pathway (198503); Epstein-Barr Virus Infection Pathway (585584); Epstein-Barr Virus Infection Pathway (587115); MicroRNAs In Cancer Pathway (852719); MicroRNAs In Cancer Pathway (852928); Spinal Cord Injury Pathway (739006); Striated Muscle Contraction Pathway (198436)

class-III intermediate filament of the cytoskeleton; along with desmin (the subunit specifically expressed in muscle), vimentin is specific for mesenchymal tissue [RGD, Feb 2006]

Function: Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.1 PublicationManual assertion based on experiment in:Ref.6

24-Strip-Wells (LIMIT 1)

240 USD

48-Strip-Wells

550

96-Strip-Wells

795

5x96-Strip-Wells

2890

10x96-Strip-Wells

5465