ELISA/assay

Mouse intestinal fatty acid binding protein, iFABP ELISA Kit

MBS702875

48-Strip-Wells

510 USD

ELISA Kit

Mouse intestinal fatty acid binding protein, iFABP ELISA Kit

intestinal fatty acid binding protein, iFABP

Mouse intestinal fatty acid binding protein; iFABP ELISA Kit; FABPI; I-FABP; MGC133132; intestinal fatty acid binding protein 2; FABP2; intestinal fatty acid binding protein; iFABP

fatty acid-binding protein, intestinal; Fatty acid-binding protein, intestinal; fatty acid-binding protein, intestinal; FABPI; I-FABP; fatty acid-binding protein 2; intestinal-type fatty acid-binding protein; Fatty acid-binding protein 2; Intestinal-type fatty acid-binding protein; I-FABP

iFABP

FABP2; FABP2; FABPI; I-FABP

FABPI_BOVIN

Mouse

132

This assay has high sensitivity and excellent specificity for detection of mouse iFABP. No significant cross-reactivity or interference between mouse iFABP and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, cell culture supernates and tissue homogenates. Assay Type: Sandwich. Detection Range: 6.25 pg/ml - 400 pg/ml. Sensitivity: The minimum detectable dose of mouse iFABP is typically less than 1.56 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for iFABP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any iFABP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for iFABP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of iFABP bound in the initial step. The color development is stopped and the intensity of the color is measured.

70778942

NP_001020503.1

NM_001025332.1

Q56JX9

15,036 Da

Fat Digestion And Absorption Pathway (194380); Fat Digestion And Absorption Pathway (194324); PPAR Signaling Pathway (84281); PPAR Signaling Pathway (450)

Function: FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. FABP2 is probably involved in triglyceride-rich lipoprotein synthesis. Binds saturated long-chain fatty acids with a high affinity, but binds with a lower affinity to unsaturated long-chain fatty acids. FABP2 may also help maintain energy homeostasis by functioning as a lipid sensor

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800