catalog number :
MBS702873
products type :
ELISA Kit
products full name :
Horse Beta-Endorphin, beta-EP ELISA Kit
products short name :
Beta-Endorphin, beta-EP
products name syn :
Horse Beta-Endorphin; beta-EP ELISA Kit; Beta-Endorphin; beta-EP
other names :
Beta-endorphin; Beta-endorphin
products gene name :
beta-EP
uniprot entry name :
COLI_CAMDR
specificity :
This assay has high sensitivity and excellent specificity for detection of horse beta-EP. No significant cross-reactivity or interference between horse beta-EP and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma. Assay Type: Sandwich. Detection Range: 3.12 pg/ml-200 pg/ml. Sensitivity: The minimum detectable dose of horse beta-EP is typically less than 0.78 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for beta-EP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any beta-EP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for beta-EP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of beta-EP bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi mol weight :
3,438 Da
uniprot summary :
Function: Beta-endorphin and Met-enkephalin are endogenous opiates.
size4 :
10x96-Strip-Wells