ELISA/assay

Human heme oxygenase 2, HO-2 ELISA Kit

MBS702870

48-Strip-Wells

565 USD

ELISA Kit

Human heme oxygenase 2, HO-2 ELISA Kit

heme oxygenase (decycling) 2

Human heme oxygenase 2; HO-2 ELISA Kit; HO-2; heme oxygenase (decyclizing) 2; heme oxygenase (decycling) 2

heme oxygenase 2 isoform b; Heme oxygenase 2; heme oxygenase 2; heme oxygenase (decycling) 2

HMOX2

HMOX2; HMOX2; HO-2; HO2; HO-2

HMOX2_HUMAN

Human

316

This assay has high sensitivity and excellent specificity for detection of Human HMOX2. No significant cross-reactivity or interference between Human HMOX2 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to HO-2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for HO-2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain HO-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of HO-2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

187761307

NP_001120676.1

NM_001127204.1

P30519

36,033 Da

Heme Degradation Pathway (160971); Iron Uptake And Transport Pathway (187191); Metabolism Pathway (477135); Metabolism Of Porphyrins Pathway (106325); Mineral Absorption Pathway (212237); Mineral Absorption Pathway (212220); Porphyrin And Chlorophyll Metabolism Pathway (83021); Porphyrin And Chlorophyll Metabolism Pathway (407); Transmembrane Transport Of Small Molecules Pathway (106572); Heme Degradation Pathway (545346)

Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. Several alternatively spliced transcript variants encoding three different isoforms have been found for this gene. [provided by RefSeq, Oct 2013]

Function: Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Heme oxygenase 2 could be implicated in the production of carbon monoxide in brain where it could act as a neurotransmitter. Catalytic activity: Protoheme + 3 AH2 + 3 O2 = biliverdin + Fe2+ + CO + 3 A + 3 H2O.

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150