ELISA/assay

Mouse growth hormone, GH ELISA Kit

MBS702867

48-Strip-Wells

590 USD

ELISA Kit

Mouse growth hormone, GH ELISA Kit

growth hormone 1

Mouse growth hormone; GH ELISA KIT; GH; GH-N; GHN; IGHD1B; hGH-N; pituitary growth hormone; growth hormone 1

somatotropin; Somatotropin; somatotropin; growth hormone; Growth hormone

GH1

Gh; Gh1; Gh1; Gh

SOMA_MOUSE

Mouse

216

This assay has high sensitivity and excellent specificity for detection of mouse GH. No significant cross-reactivity or interference between mouse GH and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, cell culture supernates, Cell lysates. Assay Type: Sandwich. Detection Range: 3.12 pg/ml -200 pg/ml. Sensitivity: The minimum detectable dose of mouse GH is typically less than 0.78 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GH is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GH bound in the initial step. The color development is stopped and the intensity of the color is measured.

6679997

NP_032143.1

NM_008117.3

P06880

24,716 Da

Adipogenesis Pathway (198299); Cytokine Signaling In Immune System Pathway (970814); Growth Hormone Receptor Signaling Pathway (1001294); Immune System Pathway (971216); Jak-STAT Signaling Pathway (83274); Jak-STAT Signaling Pathway (488); Metabolism Of Proteins Pathway (970583); Neuroactive Ligand-receptor Interaction Pathway (83250); Neuroactive Ligand-receptor Interaction Pathway (462); Nuclear Signaling By ERBB4 Pathway (1000926)

Function: Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.

48-Strip-Wells

590 USD

96-Strip-Wells

850

5x96-Strip-Wells

2880

10x96-Strip-Wells

5440