ELISA/assay

Human Alpha1 Antichymotrypsin, AACT ELISA Kit

MBS702863

48-Strip-Wells

510 USD

ELISA Kit

Human Alpha1 Antichymotrypsin, AACT ELISA Kit

serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin) , member 3

Human Alpha1 Antichymotrypsin; AACT ELISA Kit; AACT; ACT; GIG24; GIG25; MGC88254; alpha-1-antichymotrypsin; growth-inhibiting protein 24; growth-inhibiting protein 25; serine (or cysteine) proteinase inhibitor; clade A; member 3; serpin peptidase in; serpin peptidase inhibitor; clade A (alpha-1 antiproteinase; antitrypsin); member 3

alpha-1-antichymotrypsin; Alpha-1-antichymotrypsin; alpha-1-antichymotrypsin; serpin A3; growth-inhibiting protein 24; growth-inhibiting protein 25; cell growth-inhibiting gene 24/25 protein; serine (or cysteine) proteinase inhibitor, clade A, member 3; serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 3; Cell growth-inhibiting gene 24/25 protein; Serpin A3Cleaved into the following chain:Alpha-1-antichymotrypsin His-Pro-less

SERPINA3

SERPINA3; SERPINA3; ACT; AACT; GIG24; GIG25; AACT; GIG24; GIG25; ACT

AACT_HUMAN

Human

423

This assay has high sensitivity and excellent specificity for detection of human AACT. No significant cross-reactivity or interference between human AACT and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.312 ug/ml -20 ug/ml. Sensitivity: 0.078 ug/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for AACT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AACT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AACT is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AACT bound in the initial step. The color development is stopped and the intensity of the color is measured.

50659080

NP_001076.2

NM_001085.4

P01011

47,651 Da

Selenium Pathway (198825)

The protein encoded by this gene is a plasma protease inhibitor and member of the serine protease inhibitor class. Polymorphisms in this protein appear to be tissue specific and influence protease targeting. Variations in this protein's sequence have been implicated in Alzheimer's disease, and deficiency of this protein has been associated with liver disease. Mutations have been identified in patients with Parkinson disease and chronic obstructive pulmonary disease. [provided by RefSeq, Jul 2008]

Function: Although its physiological function is unclear, it can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2.1 PublicationManual assertion based on experiment in:Ref.14

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800