ELISA/assay

Rat Soluble protein-100B, S-100B ELISA Kit

MBS702860

48-Strip-Wells

605 USD

ELISA Kit

Rat Soluble protein-100B, S-100B ELISA Kit

S100 calcium binding protein B

Rat Soluble protein-100B; S-100B ELISA Kit; NEF; S100; S100beta; OTTHUMP00000174958; S-100 calcium-binding protein; beta chain; S100 beta; S100 calcium binding protein; beta (neural) ; S100 calcium-binding protein; beta; S100 calcium-binding protei; S100 calcium binding protein B

protein S100-B; Protein S100-B; protein S100-B; S-100 protein beta chain; S-100 protein subunit beta; S100 calcium-binding protein B; S-100 calcium-binding protein beta subunit; S-100 protein beta chain; S-100 protein subunit beta; S100 calcium-binding protein B

S100B

S100B; S100B

S100B_RABIT

Rat

92

This assay has high sensitivity and excellent specificity for detection of Rat S100B. No significant cross-reactivity or interference between Rat S100B and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 3.12 pg/ml-200 pg/ml. Sensitivity: 0.78 pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for S-100B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any S-100B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for S-100B is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of S-100B bound in the initial step. The color development is stopped and the intensity of the color is measured.

164414447

NP_001076199.1

NM_001082730.1

Q6YNR6

10,713 Da

Activated TLR4 Signalling Pathway (106400); Advanced Glycosylation Endproduct Receptor Signaling Pathway (187092); Cytosolic Sensors Of Pathogen-associated DNA Pathway (576255); DAI Mediated Induction Of Type I IFNs Pathway (576256); Immune System Pathway (106386); Innate Immune System Pathway (106387); MyD88 Cascade Initiated On Plasma Membrane Pathway (205107); MyD88 Dependent Cascade Initiated On Endosome Pathway (187081); MyD88-independent Cascade Initiated On Plasma Membrane Pathway (106401); MyD88:Mal Cascade Initiated On Plasma Membrane Pathway (106390)

Function: Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. Could assist ATAD3A cytoplasmic processing, preventing aggregation and favoring mitochondrial localization. May mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity

48-Strip-Wells

605 USD

96-Strip-Wells

870

5x96-Strip-Wells

2950

10x96-Strip-Wells

5535