catalog number :
MBS702851
products type :
ELISA Kit
products full name :
Bovine transforming growth factors beta2, TGFbeta2 ELISA Kit
products short name :
transforming growth factor, beta 2
products name syn :
Bovine transforming growth factors beta2; TGFbeta2 ELISA Kit; MGC116892; TGF-beta2; ; transforming growth factor; beta 2
other names :
transforming growth factor beta-2; Transforming growth factor beta-2; transforming growth factor beta-2; TGF-beta-2; milk growth factor; Milk growth factor; MGF
products gene name :
TGFB2
other gene names :
TGFB2; TGFB2; MGF; TGF-beta-2; MGF
uniprot entry name :
TGFB2_BOVIN
specificity :
This assay has high sensitivity and excellent specificity for detection of bovine TGFbeta2. No significant cross-reactivity or interference between bovine TGFbeta2 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Competitive. Detection Range: 6.25 ng/ml -100 ng/ml. Sensitivity: The minimum detectable dose of bovine TGFbeta2 is typically less than 6.25 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest bovine TGFbeta2 concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Priciple of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to TGFbeta2. Standards or samples are added to the appropriate microtiter plate wells with Biotin-conjugated TGFbeta2. A competitive inhibition reaction is launched between TGFbeta2 (Standards or samples) and Biotin-conjugated TGFbeta2 with the pre-coated antibody specific for TGFbeta2. The more amount of TGFbeta2 in samples, the less antibody bound by Biotin-conjugated TGFbeta2. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of TGFbeta2 in the sample. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001106723.1
ncbi gb acc num :
NM_001113252.1
ncbi mol weight :
47,692 Da
ncbi pathways :
Amoebiasis Pathway (167315); Amoebiasis Pathway (167191); Cell Cycle Pathway (84293); Cell Cycle Pathway (463); Chagas Disease (American Trypanosomiasis) Pathway (147808); Chagas Disease (American Trypanosomiasis) Pathway (147795); Chronic Myeloid Leukemia Pathway (84351); Chronic Myeloid Leukemia Pathway (528); Colorectal Cancer Pathway (84341); Colorectal Cancer Pathway (518)
uniprot summary :
Function: TGF-beta 2 has suppressive effects on interleukin-2 dependent T-cell growth.
size4 :
10x96-Strip-Wells