Human Matrix metalloproteinase 8/Neutrophil collagenase, MMP-8 ELISA Kit | MyBioSource MBS702847 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Matrix metalloproteinase 8/Neutrophil collagenase, MMP-8 ELISA Kit

catalog :

MBS702847

quantity :

48-Strip-Wells

price :

335 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS702847

products type :

ELISA Kit

products full name :

Human Matrix metalloproteinase 8/Neutrophil collagenase, MMP-8 ELISA Kit

products short name :

matrix metallopeptidase 8 (neutrophil collagenase)

products name syn :

Human Matrix metalloproteinase 8/Neutrophil collagenase; MMP-8 ELISA kit; CLG1; HNC; PMNL-CL; PMNL collagenase; matrix metalloproteinase 8; matrix metalloproteinase 8 (neutrophil collagenase) ; matrix metallopeptidase 8 (neutrophil collagenase)

other names :

neutrophil collagenase preproprotein; Neutrophil collagenase; neutrophil collagenase; PMNL collagenase; matrix metalloproteinase-8; matrix metalloproteinase 8 (neutrophil collagenase); matrix metallopeptidase 8 (neutrophil collagenase); Matrix metalloproteinase-8; MMP-8; PMNL collagenase; PMNL-CL

products gene name :

MMP8

other gene names :

MMP8; MMP8; HNC; CLG1; MMP-8; PMNL-CL; CLG1; MMP-8; PMNL-CL

uniprot entry name :

MMP8_HUMAN

reactivity :

Human

sequence length :

467

specificity :

This assay has high sensitivity and excellent specificity for detection of human MMP-8. No significant cross-reactivity or interference between human MMP-8 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma. Assay Type: Sandwich. Detection Range: 62.5 pg/ml - 4000 pg/ml. Sensitivity: The minimum detectable dose of human MMP-8 is typically less than 15.6 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MMP-8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MMP-8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MMP-8 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MMP-8 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

4505221

ncbi acc num :

NP_002415.1

ncbi gb acc num :

NM_002424.2

uniprot acc num :

P22894

ncbi mol weight :

53,412 Da

ncbi pathways :

Activation Of Matrix Metalloproteinases Pathway (576264); Collagen Degradation Pathway (730309); Degradation Of The Extracellular Matrix Pathway (576263); Extracellular Matrix Organization Pathway (576262); Matrix Metalloproteinases Pathway (198900)

ncbi summary :

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the enzyme encoded by this gene is stored in secondary granules within neutrophils and is activated by autolytic cleavage. Its function is degradation of type I, II and III collagens. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq, Jul 2008]

uniprot summary :

Function: Can degrade fibrillar type I, II, and III collagens. Catalytic activity: Cleavage of interstitial collagens in the triple helical domain. Unlike EC 3.4.24.7, this enzyme cleaves type III collagen more slowly than type I. Cofactor: Binds 3 calcium ions per subunit. Enzyme regulation: Cannot be activated without removal of the activation peptide.

size1 :

48-Strip-Wells

price1 :

335 USD

size2 :

96-Strip-Wells

price2 :

460

size3 :

5x96-Strip-Wells

price3 :

1725

size4 :

10x96-Strip-Wells

price4 :

3200

more info or order :

MyBioSource product webpage