catalog number :
MBS702834
products type :
ELISA Kit
products full name :
Rat alpha2 macroglobulin, alpha2 MG ELISA Kit
products short name :
[alpha-2-macroglobulin]
products name syn :
[Rat alpha2 macroglobulin; alpha2 MG ELISA Kit; CPAMD5; DKFZp779B086; FWP007; S863-7; ; alpha-2-macroglobulin]
other names :
[alpha-2-macroglobulin; Alpha-2-macroglobulin; alpha-2-macroglobulin; alpha-2-M; alpha-2-macroglobulin-P; alpha-2-macroglobulin]
products gene name :
[A2M]
other gene names :
[A2m; A2m; Mam; A2m1; A2maa; A2MAC1; A2m1; Alpha-2-M]
uniprot entry name :
A2MG_RAT
specificity :
This assay has high sensitivity and excellent specificity for detection of rat alpha2 MG. No significant cross-reactivity or interference between rat alpha2 MG and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, plasma. Assay Type: Quantitative Competitive. Detection Range: 3.125 ng/ml-800 ng/ml. Sensitivity: <3.125 ng/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with rat alpha2 MG. Standards and samples are pipetted into the wells with a Horseradish Peroxidase (HRP) conjugated antibody specific for rat alpha2 MG. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in opposite to the amount of rat alpha2 MG in samples. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_036620.2
ncbi gb acc num :
NM_012488.2
ncbi mol weight :
163,785 Da
ncbi pathways :
Complement And Coagulation Cascades Pathway (198461); Complement And Coagulation Cascades Pathway (83465); Complement And Coagulation Cascades Pathway (484); Degradation Of The Extracellular Matrix Pathway (1009936); Extracellular Matrix Organization Pathway (1009923); Formation Of Fibrin Clot (Clotting Cascade) Pathway (1010914); HDL-mediated Lipid Transport Pathway (1010688); Hemostasis Pathway (1010891); Intrinsic Pathway (1010916); Lipid Digestion, Mobilization, And Transport Pathway (1010683)
ncbi summary :
plays a role in acute phase response; activated form induces a decrease in N-methyl-D-aspartate (NMDA) mediated calcium signaling [RGD, Feb 2006]
uniprot summary :
Function: Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.
size1 :
24-Strip-Wells (LIMIT 1)
size5 :
10x96-Strip-Wells