catalog number :
MBS702832
products type :
ELISA Kit
products full name :
Rat apolipoprotein B100, Apo-B100 ELISA Kit
products short name :
apolipoprotein B (including Ag (x) antigen)
products name syn :
Rat apolipoprotein B100 (Apo-B100) ELISA Kit; FLDB; LDLCQ4; apoB-100; apoB-48; apolipoprotein B; apolipoprotein B48; Rat apolipoprotein B100 (Apo-B100)
other names :
Apolipoprotein B, partial; Apolipoprotein B
products gene name :
APOB
uniprot entry name :
APOB_RABIT
specificity :
This assay has high sensitivity and excellent specificity for detection of rat Apo-B100. No significant cross-reactivity or interference between rat Apo-B100 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Competitive. Detection Range: 0.313 mug/ml -20 mug/ml. Sensitivity: The minimum detectable dose of rat Apo-B100 is typically less than 0.342 mug/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Apo-B100. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for Apo-B100. The competitive inhibition reaction is launched between with pre-coated Apo-B100 and Apo-B100 in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of Apo-B100 in the samples. The color development is stopped and the intensity of the color is measured.
ncbi mol weight :
15,664 Da
ncbi pathways :
Cell Surface Interactions At The Vascular Wall Pathway (106062); Chylomicron-mediated Lipid Transport Pathway (106157); FOXA1 Transcription Factor Network Pathway (137979); Fat Digestion And Absorption Pathway (194385); Fat Digestion And Absorption Pathway (194324); Hemostasis Pathway (106028); LDL-mediated Lipid Transport Pathway (106159); Lipid Digestion, Mobilization, And Transport Pathway (106111); Lipoprotein Metabolism Pathway (106156); Metabolism Pathway (477135)
uniprot summary :
Function: Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.
size3 :
10x96-Strip-Wells