catalog number :
MBS702823
products type :
ELISA Kit
products full name :
Human leucocyte antigen B27, HLA-B27 ELISA Kit
products short name :
leucocyte antigen B27, HLA-B27
products name syn :
Human leucocyte antigen B27; HLA-B27 ELISA Kit; leucocyte antigen B27; HLA-B27
other names :
HLA-B27, partial; HLA class I histocompatibility antigen, B-27 alpha chain; MHC class I antigen B*27
products gene name :
HLA-B27
other gene names :
HLA-B; HLAB
uniprot entry name :
1B27_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of Human HLA-B27. No significant cross-reactivity or interference between Human HLA-B27 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, urine, cerebrospinal fluid (CSF). Detection Range: 0.312 ng/ml-20 ng/ml. Sensitivity: 0.078 ng/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul
products description :
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to HLA-B27. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for HLA-B27 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain HLA-B27, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of HLA-B27 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ncbi acc num :
CAA27578.1
ncbi mol weight :
40,428 Da
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)
uniprot summary :
Function: Involved in the presentation of foreign antigens to the immune system.
size4 :
10x96-Strip-Wells