ELISA/assay

Dog matrix metalloproteinase 9/Gelatinase B, MMP-9 ELISA Kit

MBS702817

48-Strip-Wells

605 USD

ELISA Kit

Dog matrix metalloproteinase 9/Gelatinase B, MMP-9 ELISA Kit

matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)

Dog matrix metalloproteinase 9/Gelatinase B; MMP-9 ELISA Kit; CLG4B; GELB; MANDP2; MMP-9; macrophage gelatinase; matrix metalloproteinase 9; matrix metalloproteinase 9 (gelatinase B; 92kDa gelatinase; 92kDa type IV collagenase) ; type V collagenase; matrix metallopeptidase 9 (gelatinase B; 92kDa gelatinase; 92kDa type IV collagenase)

Matrix metalloproteinase-9; Matrix metalloproteinase-9; matrix metalloproteinase-9; GELB; MMP-9; gelatinase B; 92 kDa gelatinase; type IV collagenase MMP-9; 92 kDa type IV collagenase; matrix metalloproteinase 9; 92 kDa gelatinase; 92 kDa type IV collagenase; Gelatinase B; GELB

MMP9

MMP9; MMP9; MMP-9; GELB

MMP9_CANFA

Dog

704

This assay has high sensitivity and excellent specificity for detection of dog MMP-9. No significant cross-reactivity or interference between dog MMP-9 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma. Assay Type: Sandwich. Detection Range: 12.5 ng/ml -800 ng/ml. Sensitivity: 3.12 ng/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MMP-9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MMP-9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MMP-9 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MMP-9 bound in the initial step. The color development is stopped and the intensity of the color is measured.

14917012

O18733.2

O18733

78,123 Da

Activation Of Matrix Metalloproteinases Pathway (1016248); Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway (1016237); Axon Guidance Pathway (1016806); Bladder Cancer Pathway (84161); Bladder Cancer Pathway (527); Collagen Degradation Pathway (1016249); Collagen Formation Pathway (1016235); Degradation Of The Extracellular Matrix Pathway (1016247); Developmental Biology Pathway (1016805); EPH-Ephrin Signaling Pathway (1016833)

Function: Could play a role in bone osteoclastic resorption. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments . Catalytic activity: Cleavage of gelatin types I and V and collagen types IV and V. Cofactor: Binds 2 zinc ions per subunit

48-Strip-Wells

605 USD

96-Strip-Wells

870

5x96-Strip-Wells

2950

10x96-Strip-Wells

5535