Human protein, peptidylprolyl cis/trans isomeraseNIMA-interacting 1, PIN1 ELISA Kit | MyBioSource MBS702800 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human protein, peptidylprolyl cis/trans isomeraseNIMA-interacting 1, PIN1 ELISA Kit

catalog :

MBS702800

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS702800

products type :

ELISA Kit

products full name :

Human protein, peptidylprolyl cis/trans isomeraseNIMA-interacting 1, PIN1 ELISA Kit

products short name :

peptidylprolyl cis/trans isomerase, NIMA-interacting 1

products name syn :

Human protein (peptidylprolyl cis/trans isomerase) NIMA-interacting 1; PIN1 ELISA Kit; DOD; UBL5; peptidyl-prolyl cis/trans isomerase; NIMA-interacting; prolyl isomerase; protein (peptidyl-prolyl cis/trans isomerase) NIMA-interacting 1; protein (peptidylprolyl cis/trans isomerase) NIMA-i; peptidylprolyl cis/trans isomerase; NIMA-interacting 1

other names :

peptidyl-prolyl cis-trans isomerase NIMA-interacting 1; Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1; peptidyl-prolyl cis-trans isomerase NIMA-interacting 1; PPIase Pin1; rotamase Pin1; protein (peptidyl-prolyl cis/trans isomerase) NIMA-interacting 1; peptidylprolyl cis/trans isomerase, NIMA-interacting 1; Peptidyl-prolyl cis-trans isomerase Pin1; PPIase Pin1; Rotamase Pin1

products gene name :

PIN1

other gene names :

PIN1; PIN1; DOD; UBL5; PPIase Pin1

uniprot entry name :

PIN1_HUMAN

reactivity :

Human

sequence length :

163

specificity :

This assay has high sensitivity and excellent specificity for detection of human PIN1. No significant cross-reactivity or interference between human PIN1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, urine, tissue homogenates. Assay Type: Sandwich. Detection Range: 1.56 pg/ml -100 pg/ml. Sensitivity: The minimum detectable dose of human PIN1 is typically less than 0.39 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PIN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PIN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PIN1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PIN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

5453898

ncbi acc num :

NP_006212.1

ncbi gb acc num :

NM_006221.3

uniprot acc num :

Q13526

ncbi mol weight :

18,243 Da

ncbi pathways :

Antiviral Mechanism By IFN-stimulated Genes Pathway (530760); C-MYC Pathway (169344); Cytokine Signaling In Immune System Pathway (366171); ISG15 Antiviral Mechanism Pathway (530761); Immune System Pathway (106386); Innate Immune System Pathway (106387); Interferon Signaling Pathway (187103); Negative Regulators Of RIG-I/MDA5 Signaling Pathway (187102); RIG-I-like Receptor Signaling Pathway (117292); RIG-I-like Receptor Signaling Pathway (116128)

ncbi summary :

Peptidyl-prolyl cis/trans isomerases (PPIases) catalyze the cis/trans isomerization of peptidyl-prolyl peptide bonds. This gene encodes one of the PPIases, which specifically binds to phosphorylated ser/thr-pro motifs to catalytically regulate the post-phosphorylation conformation of its substrates. The conformational regulation catalyzed by this PPIase has a profound impact on key proteins involved in the regulation of cell growth, genotoxic and other stress responses, the immune response, induction and maintenance of pluripotency, germ cell development, neuronal differentiation, and survival. This enzyme also plays a key role in the pathogenesis of Alzheimer's disease and many cancers. Multiple alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Jun 2011]

uniprot summary :

Function: Essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Displays a preference for an acidic residue N-terminal to the isomerized proline bond. Catalyzes pSer/Thr-Pro cis/trans isomerizations. Down-regulates kinase activity of BTK. Can transactivate multiple oncogenes and induce centrosome amplification, chromosome instability and cell transformation. Required for the efficient dephosphorylation and recycling of RAF1 after mitogen activation. Binds and targets PML and BCL6 for degradation in a phosphorylation-dependent manner.5 PublicationsManual assertion based on experiment in:Ref.8. Catalytic activity: Peptidylproline (omega=180) = peptidylproline (omega=0).

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage