Human cardiac isoform of Tropnin T, cTnT ELISA Kit | MyBioSource MBS702796 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human cardiac isoform of Tropnin T, cTnT ELISA Kit

catalog :

MBS702796

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS702796

products type :

ELISA Kit

products full name :

Human cardiac isoform of Tropnin T, cTnT ELISA Kit

products short name :

troponin T type 2 (cardiac)

products name syn :

Human cardiac isoform of Tropnin T; cTnT ELISA Kit; CMH2; CMPD2; MGC3889; RCM3; TnTC; cTnT; OTTHUMP00000033864; OTTHUMP00000033867; cardiomyopathy; dilated 1D (autosomal dominant) ; cardiomyopathy; hypertrophic 2; troponin T type 2; cardiac; troponin T; ca; troponin T type 2 (cardiac)

other names :

troponin T, cardiac muscle isoform 1; Troponin T, cardiac muscle; troponin T, cardiac muscle; troponin T2, cardiac; cardiomyopathy, hypertrophic 2; cardiomyopathy, dilated 1D (autosomal dominant); troponin T type 2 (cardiac); Cardiac muscle troponin T; cTnT

products gene name :

TNNT2

other gene names :

TNNT2; TNNT2; CMH2; RCM3; TnTC; cTnT; CMD1D; CMPD2; LVNC6; TnTc; cTnT

uniprot entry name :

TNNT2_HUMAN

reactivity :

Human

sequence length :

295

specificity :

This assay has high sensitivity and excellent specificity for detection of human cTnT. No significant cross-reactivity or interference between human cTnT and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 62.5 pg/ml -4000 pg/ml. Sensitivity: The minimum detectable dose of human cTnT is typically less than 15.6 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for cTnT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any cTnT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for cTnT is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of cTnT bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

48255877

ncbi acc num :

NP_000355.2

ncbi gb acc num :

NM_000364.3

uniprot acc num :

P45379

ncbi mol weight :

35,924 Da

ncbi pathways :

Adrenergic Signaling In Cardiomyocytes Pathway (908257); Adrenergic Signaling In Cardiomyocytes Pathway (909696); Cardiac Progenitor Differentiation Pathway (712094); Cardiac Muscle Contraction Pathway (93344); Cardiac Muscle Contraction Pathway (93992); Dilated Cardiomyopathy Pathway (121494); Dilated Cardiomyopathy Pathway (121285); Hypertrophic Cardiomyopathy (HCM) Pathway (114229); Hypertrophic Cardiomyopathy (HCM) Pathway (106591); Muscle Contraction Pathway (106261)

ncbi summary :

The protein encoded by this gene is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for this gene undergo alternative splicing that results in many tissue-specific isoforms, however, the full-length nature of some of these variants has not yet been determined. [provided by RefSeq, Jul 2008]

uniprot summary :

Function: Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage