Human 8-oxoguanine DNA glycosydase, hOGG1 ELISA Kit | MyBioSource MBS702793 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human 8-oxoguanine DNA glycosydase, hOGG1 ELISA Kit

catalog :

MBS702793

quantity :

48-Strip-Wells

price :

590 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS702793

products type :

ELISA Kit

products full name :

Human 8-oxoguanine DNA glycosydase, hOGG1 ELISA Kit

products short name :

8-oxoguanine DNA glycosylase

products name syn :

Human 8-oxoguanine DNA glycosydase (hOGG1) ELISA kit; HMMH; HOGG1; MUTM; OGH1; 8-hydroxyguanine DNA glycosylase; 8-oxoguanine DNA-glycosylase 1; AP lyase; DNA-apurinic or apyrimidinic site lyase; 8-oxoguanine DNA glycosylase

other names :

N-glycosylase/DNA lyase isoform 1a; N-glycosylase/DNA lyase; N-glycosylase/DNA lyase; AP lyase; OGG1 type 1f; 8-hydroxyguanine DNA glycosylase; DNA-apurinic or apyrimidinic site lyase; 8-oxoguanine DNA glycosylase

products gene name :

OGG1

other gene names :

OGG1; OGG1; HMMH; MUTM; OGH1; HOGG1; MMH; MUTM; OGH1; AP lyase

uniprot entry name :

OGG1_HUMAN

reactivity :

Human

sequence length :

345

specificity :

This assay has high sensitivity and excellent specificity for detection of human hOGG1. No significant cross-reactivity or interference between human hOGG1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, tissue homogenates, cell lysates. Assay Type: Sandwich. Detection Range: 0.312 ng/ml -20 ng/ml. Sensitivity: 0.078 ng/ml.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for hOGG1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any hOGG1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for hOGG1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of hOGG1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

4505495

ncbi acc num :

NP_002533.1

ncbi gb acc num :

NM_002542.5

uniprot acc num :

O15527

ncbi mol weight :

38,782 Da

ncbi pathways :

Base Excision Repair Pathway (105838); Base Excision Repair Pathway (83043); Base Excision Repair Pathway (451); Base-Excision Repair, AP Site Formation Pathway (105839); Base-free Sugar-phosphate Removal Via The Single-nucleotide Replacement Pathway (105848); Cleavage Of The Damaged Purine Pathway (105842); DNA Repair Pathway (105837); Depurination Pathway (105840); Displacement Of DNA Glycosylase By APE1 Pathway (105849); Recognition And Association Of DNA Glycosylase With Site Containing An Affected Purine Pathway (105841)

ncbi summary :

This gene encodes the enzyme responsible for the excision of 8-oxoguanine, a mutagenic base byproduct which occurs as a result of exposure to reactive oxygen. The action of this enzyme includes lyase activity for chain cleavage. Alternative splicing of the C-terminal region of this gene classifies splice variants into two major groups, type 1 and type 2, depending on the last exon of the sequence. Type 1 alternative splice variants end with exon 7 and type 2 end with exon 8. All variants share the N-terminal region in common, which contains a mitochondrial targeting signal that is essential for mitochondrial localization. Many alternative splice variants for this gene have been described, but the full-length nature for every variant has not been determined. [provided by RefSeq, Aug 2008]

uniprot summary :

Function: DNA repair enzyme that incises DNA at 8-oxoG residues. Excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine (FAPY) from damaged DNA. Has a beta-lyase activity that nicks DNA 3' to the lesion. Catalytic activity: The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.

size1 :

48-Strip-Wells

price1 :

590 USD

size2 :

96-Strip-Wells

price2 :

850

size3 :

5x96-Strip-Wells

price3 :

2880

size4 :

10x96-Strip-Wells

price4 :

5440

more info or order :

MyBioSource product webpage