ELISA/assay

Mouse Substance P, SP ELISA Kit

MBS702782

24-Strip-Wells (LIMIT 1)

240 USD

ELISA Kit

Mouse Substance P, SP ELISA Kit

[Substance P, SP]

[Mouse Substance P; SP ELISA Kit; Substance P; SP]

[substance P, partial; Substance P; Substance P]

[SP]

D0PTV7_CRYPR

Mouse

49

This assay has high sensitivity and excellent specificity for detection of Mouse SP. No significant cross-reactivity or interference between Mouse SP and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, plasma, cell culture supernates and tissue homogenates. Detection Range: 3.12 pg/ml-200 pg/ml. Sensitivity: 0.78 pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

Introduction: In the field of neuroscience, substance P (SP) is a neuropeptide: an undecapeptide that functions as a neurotransmitter and as a neuromodulator. It belongs to the tachykinin neuropeptide family. Substance P and its closely related neuropeptide neurokinin A (NKA) are produced from a polyprotein precursor after differential splicing of the preprotachykinin A gene. The deduced amino acid sequence of substance P is as follows: Arg Pro Lys Pro Gln Gln Phe Phe Gly Leu Met. Substance P is released from the terminals of specific sensory nerves, it is found in the brain and spinal cord, and is associated with inflammatory processes and pain. Substance P is an important element in pain perception. The sensory function of substance P is thought to be related to the transmission of pain information into the central nervous system. Substance P coexists with the excitatory neurotransmitter glutamate in primary afferents that respond to painful stimulation. SP has been associated with the regulation of mood disorders, anxiety, stress, reinforcement, neurogenesis, respiratory rhythm, neurotoxicity, nausea/emesis, pain and nociception. Substance P and other sensory neuropeptides can be released from the peripheral terminals of sensory nerve fibers in the skin, muscle and joints. It is proposed that this release is involved in neurogenic inflammation which is a local inflammatory response to certain types of infection or injury. The regulatory function of SP also involves the regulation of its high-affinity receptor, NK-1. Substance P receptor antagonists may have important therapeutic applications in the treatment of a variety of stress-related illnesses, in addition to their potential as analgesics. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Substance P. Standards or samples are then added to the appropriate microtiter plate wells with a biotin conjugated antibody preparation specific for Substance P and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain Substance P, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Substance P in the samples is then determined by comparing the O.D. of the samples to the standard curve.

224037302

ACN37865.1

D0PTV7

5,522 Da

24-Strip-Wells (LIMIT 1)

240 USD

48-Strip-Wells

320

96-Strip-Wells

445

5x96-Strip-Wells

1880

10x96-Strip-Wells

3540