ELISA/assay

Human High Molecular Weight Adiponectin, HMW Adiponectin ELISA Kit

MBS702778

48-Strip-Wells

565 USD

ELISA Kit

Human High Molecular Weight Adiponectin, HMW Adiponectin ELISA Kit

High Molecular Weight Adiponectin (HMW Adiponectin)

Human High Molecular Weight Adiponectin (HMW Adiponectin) ELISA Kit; High Molecular Weight Adiponectin (HMW Adiponectin)

HMW Adiponectin

Human

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, cell culture supernates, tissue homogenates, urine. Detection Range: 3.12ng/ml-200 ng/ml. Sensitivity: 0.78 ng/ml

Detection Wavelength: 450 nm. Sample Volume: 50-100ul

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to HMW Adiponectin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for HMW Adiponectin and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain HMW Adiponectin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of HMW Adiponectin in the samples is then determined by comparing the O.D. of the samples to the standard curve.

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150