ELISA/assay

Human Urotensin II ELISA Kit

MBS702757

96 Strip Wells

895 USD

ELISA Kit

Human Urotensin II ELISA Kit

urotensin 2

Human Urotensin II ELISA kit; PRO1068; U-II; UCN2; UII; ; urotensin 2

urotensin-2 isoform b preproprotein; Urotensin-2; urotensin-2; prepro U-II; urotensin II; urotensin 2; Urotensin II; U-II; UII

UTS2

UTS2; UTS2; UII; U-II; UCN2; PRO1068; UNQ525/PRO1068; U-II; UII

UTS2_HUMAN

Human

124

This assay has high sensitivity and excellent specificity for detection of human urotensinII. No significant cross-reactivity or interference between human urotensinII and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: For the quantitative determination of human urotensinII concentrations in serum, saliva, cerebrospinal fluid (CSF), tissue homogenates. Detection Range: 0.312ng/ml-20ng/ml.

Sensitivity: The minimum detectable dose of human urotensinIIis typically less than 0.078ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays):CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for urotensinII has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any urotensinII present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific forurotensinII is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of urotensinII bound in the initial step. The color development is stopped and the intensity of the color is measured.

5803209

NP_006777.1

NM_006786.3

O95399

14,296 Da

Class A/1 (Rhodopsin-like Receptors) Pathway 106357!!G Alpha (q) Signalling Events Pathway 106043!!GPCR Downstream Signaling Pathway 119548!!GPCR Ligand Binding Pathway 161020!!Gastrin-CREB Signalling Pathway Via PKC And MAPK 645295!!Peptide Ligand-binding Receptors Pathway 106358!!Signal Transduction Pathway 477114!!Signaling By GPCR Pathway 106356

This gene encodes a mature peptide that is an active cyclic heptapeptide absolutely conserved from lamprey to human. The active peptide acts as a vasoconstrictor and is expressed only in brain tissue. Despite the gene family name similarity, this gene is not homologous to urocortin, a member of the sauvagine/corticotropin-releasing factor/urotensin I family. Most of the proprotein is cleaved to make the mature peptide. Transcript variants encoding different preproprotein isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Function: Highly potent vasoconstrictor.

96 Strip Wells

895 USD