This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
MyBioSource
product type :
ELISA/assay
product name :
Pig Pulmonary surfactant-associated protein A, SP-A ELISA Kit
catalog :
MBS702747
quantity :
48-Strip-Wells
price :
605 USD
product information
catalog number :
MBS702747
products type :
ELISA Kit
products full name :
Pig Pulmonary surfactant-associated protein A, SP-A ELISA Kit
products short name :
surfactant protein A1
products name syn :
Pig Pulmonary surfactant-associated protein A; SP-A ELISA Kit; AC068139.6; MGC133365; PSAP; PSPA; SFTP1; SFTPA1; pulmonary surfactant apoprotein; surfactant; pulmonary-associated protein A1; surfactant; pulmonary-associated protein A1B; surfactant protein A1
other names :
pulmonary surfactant-associated protein A; Pulmonary surfactant-associated protein A; pulmonary surfactant-associated protein A; PSAP; SP-A
products gene name :
SFTPA1
other gene names :
SFTPA1; SFTPA1; PSP-A; SFTPA; SFTP1; SFTPA; PSAP; PSP-A; SP-A
uniprot entry name :
SFTPA_PIG
reactivity :
Pig
sequence length :
249
specificity :
This assay has high sensitivity and excellent specificity for detection of pig SP-A. No significant cross-reactivity or interference between pig SP-A and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 1.5 6 ng/ml -100 ng/ml. Sensitivity: The minimum detectable dose of pig SP-A is typically less than 0.39 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SP-A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SP-A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SP-A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SP-A bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi gi num :
47523604
ncbi acc num :
NP_999430.1
ncbi gb acc num :
NM_214265.1
uniprot acc num :
P49874
ncbi mol weight :
26,702 Da
ncbi pathways :
Pertussis Pathway (218109); Pertussis Pathway (218099); Phagosome Pathway (153902); Phagosome Pathway (153859)
uniprot summary :
Function: In presence of calcium ions, PSAP binds to surfactant phospholipids and contributes to lower the surface tension at the air-liquid interface in the alveoli of the mammalian lung and is essential for normal respiration.
size1 :
48-Strip-Wells
price1 :
605 USD
size2 :
96-Strip-Wells
price2 :
870
size3 :
5x96-Strip-Wells
price3 :
2950
size4 :
10x96-Strip-Wells
price4 :
5535
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

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