catalog number :
MBS702746
products type :
ELISA Kit
products full name :
Rat N-acetyl-beta-D-glucosaminidase, NAG ELISA Kit
products short name :
N-acetyl-beta-D-glucosaminidase, NAG
products name syn :
Rat N-acetyl-beta-D-glucosaminidase; NAG ELISA Kit; ENC-1AS; N-acetyl-beta-glucosaminidase; hexosaminidase B; Beta-hexosaminidase subunit beta (HEXB) ; N-acetyl-beta-D-glucosaminidase; NAG
other names :
beta-hexosaminidase subunit beta; Beta-hexosaminidase subunit beta; beta-hexosaminidase subunit beta; hexosaminidase subunit B; beta-N-acetylhexosaminidase subunit beta; N-acetyl-beta-glucosaminidase subunit beta; hexosaminidase B; Beta-N-acetylhexosaminidase subunit beta; Hexosaminidase subunit B; N-acetyl-beta-glucosaminidase subunit beta
other gene names :
Hexb; Hexb; Hexosaminidase subunit B
uniprot entry name :
HEXB_RAT
specificity :
This assay has high sensitivity and excellent specificity for detection of Rat NAG. No significant cross-reactivity or interference between Rat NAG and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, cell culture supernates, tissue homogenates, urine. Assay Type: Sandwich. Detection Range: 1.56 mIU/ml-100 mIU/ml. Sensitivity: 0.39 mIU/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NAG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NAG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NAG is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NAG bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001011946.1
ncbi gb acc num :
NM_001011946.1
ncbi mol weight :
61,527 Da
ncbi pathways :
Amino Sugar And Nucleotide Sugar Metabolism Pathway (83371); Amino Sugar And Nucleotide Sugar Metabolism Pathway (350); Glycosaminoglycan Degradation Pathway (83373); Glycosaminoglycan Degradation Pathway (355); Glycosphingolipid Biosynthesis - Ganglio Series Pathway (83389); Glycosphingolipid Biosynthesis - Ganglio Series Pathway (372); Glycosphingolipid Biosynthesis - Globo Series Pathway (83388); Glycosphingolipid Biosynthesis - Globo Series Pathway (371); Keratan Sulfate Degradation Pathway (434694); Keratan Sulfate Degradation Pathway (468269)
uniprot summary :
Function: Responsible for the degradation of GM2 gangliosides, and a variety of other molecules containing terminal N-acetyl hexosamines, in the brain and other tissues . Catalytic activity: Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides.
size4 :
10x96-Strip-Wells