ELISA/assay

Rat Endothelial nitric oxide synthase, eNOS/NOS3 ELISA Kit

MBS702741

48-Strip-Wells

565 USD

ELISA Kit

Rat Endothelial nitric oxide synthase, eNOS/NOS3 ELISA Kit

nitric oxide synthase 3 (endothelial cell)

Rat Endothelial nitric oxide synthase; eNOS/NOS3 ELISA Kit; ECNOS; eNOS; nitric oxide synthase 3; nitric oxide synthase 3 (endothelial cell)

nitric oxide synthase, endothelial; Nitric oxide synthase, endothelial; nitric oxide synthase, endothelial; cNOS; EC-NOS; NOSIII; NOS type III; endothelial NOS; constitutive NOS; endothelial nitric oxide synthase 3; nitric oxide synthase 3, endothelial cell; Constitutive NOS; cNOS; EC-NOS; Endothelial NOS; eNOS; NOS type III; NOSIII

NOS3

Nos3; Nos3; eNos; cNOS; eNOS; NOSIII

NOS3_RAT

Rat

1202

This assay has high sensitivity and excellent specificity for detection of Rat NOS3. No significant cross-reactivity or interference between Rat NOS3 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, cell culture supernates and Cell lysates. Detection Range: 15.6 uIU/ml -1000 uIU/ml. Sensitivity: 3.9 uIU/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

Introduction: Nitric oxide (NO) is produced by a group of enzymes called nitric oxide synthases (NOS). These enzymes catalyze the production of NO and L-citrulline from L-arginine, O2, and NADPH derived electrons. Mammalian systems contain three well-characterized isoforms of the enzyme: neuronal NOS (nNOS, also called NOS1), inducible NOS (iNOS or NOS2), and endothelial NOS (eNOS or NOS3). The cell types that express eNOS include vascular endothelial cells, cardiomyocytes and others. In blood vessels, NO produced by the eNOS in endothelial cells functions as a vasodilator thereby regulating blood flow and pressure. Mutant eNOS knockout mice have blood pressure that is 30% higher than wild-type littermates. Within cardiomyocytes, eNOS affects Ca2+ currents and contractility. Expression of eNOS is usually reported to be constitutive, though modest degrees of regulation occur in response to factors such as shear stress, exercise, chronic hypoxia, and heart failure. The unique N-terminal sequence of eNOS is about 70 residues long and functions to localize the enzyme to membranes. Upon myristoylation at one site and palmitoylation at two other sites within this segment, the enzyme is exclusively membrane-bound. Palmitoylation is a reversible process that is influenced by some agonists and is essential for membrane localization. Within the membrane, eNOS is targeted to the caveolae, small invaginations characterized by the presence of proteins called caveolins. These regions serve as sites for the sequestration of signaling molecules such as receptors, G proteins and protein kinases. The oxygenase domain of eNOS contains a motif that binds to caveolin-1, and calmodulin is believed to competitively displace caveolin resulting in eNOS activation. Bound calmodulin is required for activity of eNOS, and this binding occurs in response to transient increases in intracellular Ca2+. Thus, eNOS occurs at sites of signal transduction and produces short pulses of NO in response to agonists that elicit Ca2+ transients. Physiological concentrations of eNOS-derived NO are in the picomolar range . Within the cardiovascular system, eNOS generally has protective effects. Studies with nNOS and eNOS knockout mice clearly indicate that eNOS plays a protective role in cerebral is chemia by preserving cerebral blood flow. During inflammation and atherosclerosis, low concentrations of NO prevent apoptotic death of endothelial cells and preserve the integrity of the endothelial cell monolayer. NO also acts as an inhibitor of platelet aggregation, adhesion molecule expression, and vascular smooth muscle cell proliferation. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to eNOS. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for eNOS and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain eNOS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of eNOS in the samples is then determined by comparing the O.D. of the samples to the standard curve.

46402488

NP_068610.1

NM_021838.2

Q62600

133,290 Da

ACE Inhibitor Pathway (198448); Arginine And Proline Metabolism Pathway (83349); Arginine And Proline Metabolism Pathway (323); Calcium Signaling Pathway (83442); Calcium Signaling Pathway (459); Disease Pathway (1010484); Estrogen Signaling Pathway (799190); Estrogen Signaling Pathway (799197); HIF-1 Signaling Pathway (695233); Hemostasis Pathway (1010891)

enzyme that synthesizes Nitric oxide from L-arginine [RGD, Feb 2006]

Function: Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Catalytic activity: 2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O. Cofactor: Heme group. Enzyme regulation: Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150