catalog number :
MBS702723
products type :
ELISA Kit
products full name :
Mouse bone morphogenetic protein 9, BMP-9 ELISA Kit
products short name :
growth differentiation factor 2
products name syn :
Mouse bone morphogenetic protein 9; BMP-9 ELISA Kit; BMP-9; BMP9; bone morphogenetic protein 9; growth differentiation factor 2
other names :
growth/differentiation factor 2; Growth/differentiation factor 2; growth/differentiation factor 2; BMP-9; GDF-2; bone morphogenetic protein 9; growth differentiation factor 2; Bone morphogenetic protein 9; BMP-9
products gene name :
GDF2
other gene names :
Gdf2; Gdf2; Bmp9; Bmp9; GDF-2; BMP-9
uniprot entry name :
GDF2_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of Mouse GDF2. No significant cross-reactivity or interference between Mouse GDF2 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.312 pg/ml-20 pg/ml. Sensitivity: 0.078 pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for BMP-9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any BMP-9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BMP-9 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BMP-9 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_062379.3
ncbi gb acc num :
NM_019506.4
ncbi mol weight :
47,703 Da
ncbi pathways :
ALK1 Signaling Events Pathway (137968)
uniprot summary :
Function: Potent circulating inhibitor of angiogenesis. Could be involved in bone formation. Signals through the type I activin receptor ACVRL1 but not other Alks. Signaling through SMAD1 in endothelial cells requires TGF-beta coreceptor endoglin/ENG.1 PublicationManual assertion based on experiment in:Ref.6
size4 :
10x96-Strip-Wells