product summary
Loading...
company name :
MyBioSource
product type :
ELISA/assay
product name :
Rat Interleukin 1beta, IL-1beta ELISA Kit
catalog :
MBS702717
quantity :
48-Strip-Wells
price :
335 USD
more info or order :
MyBioSource product webpage
citations: 1
Reference
Giunta S, Castorina A, Marzagalli R, Szychlinska M, Pichler K, Mobasheri A, et al. Ameliorative effects of PACAP against cartilage degeneration. Morphological, immunohistochemical and biochemical evidence from in vivo and in vitro models of rat osteoarthritis. Int J Mol Sci. 2015;16:5922-44 pubmed publisher
product information
catalog number :
MBS702717
products type :
ELISA Kit
products full name :
Rat Interleukin 1beta, IL-1beta ELISA Kit
products short name :
interleukin 1, beta
products name syn :
Rat Interleukin 1beta; IL-1beta ELISA Kit; IL-1; IL1-BETA; IL1F2; catabolin; preinterleukin 1 beta; pro-interleukin-1-beta; interleukin 1; beta
other names :
interleukin-1 beta; Interleukin-1 beta; interleukin-1 beta; IL-1 beta; interleukin 1 beta; lymphocyte proliferation-potentiating factor; Lymphocyte proliferation-potentiating factor
products gene name :
IL1B
other gene names :
IL1B; IL1B; IL1-BETA; IL-1 beta
uniprot entry name :
IL1B_RABIT
reactivity :
Rat
sequence length :
268
specificity :
This assay has high sensitivity and excellent specificity for detection of rat IL-1beta. No significant cross-reactivity or interference between rat IL-1beta and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Sandwich. Detection Range: 62.5 pg/ml -4000 pg/ml. Sensitivity: The minimum detectable dose of rat IL-1beta is typically less than 15.6 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL-1beta has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-1beta present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-1beta is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-1beta bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi gi num :
126723555
ncbi acc num :
NP_001075670.1
ncbi gb acc num :
NM_001082201.1
uniprot acc num :
P14628
ncbi mol weight :
30,665 Da
ncbi pathways :
African Trypanosomiasis Pathway (194384); African Trypanosomiasis Pathway (194323); Alzheimer's Disease Pathway (83097); Alzheimer's Disease Pathway (509); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Apoptosis Pathway (83060); Apoptosis Pathway (470); Cellular Roles Of Anthrax Toxin Pathway (138076); Chagas Disease (American Trypanosomiasis) Pathway (147809)
uniprot summary :
Function: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
size1 :
48-Strip-Wells
price1 :
335 USD
size2 :
96-Strip-Wells
price2 :
460
size3 :
5x96-Strip-Wells
price3 :
1725
size4 :
10x96-Strip-Wells
price4 :
3200
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

"MyBioSource offers the best products at unbeatable prices."

Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

Would you like to receive email and e-newsletter from MyBioSource about new products, special offers and events? Please click here to join our Mailing List!