Rat calcium/calmodulin-dependent protein kinase-II, CAMK 2 ELISA Kit | MyBioSource MBS702708 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat calcium/calmodulin-dependent protein kinase-II, CAMK 2 ELISA Kit

catalog :

MBS702708

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS702708

products type :

ELISA Kit

products full name :

Rat calcium/calmodulin-dependent protein kinase-II, CAMK 2 ELISA Kit

products short name :

calcium/calmodulin-dependent protein kinase II alpha

products name syn :

Rat calcium/calmodulin-dependent protein kinase-II; CAMK 2 ELISA Kit; CAMKA; KIAA0968; CaM kinase II alpha subunit; CaM-kinase II alpha chain; CaMK-II alpha subunit; CaMKIINalpha; calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha; calcium/calmodulin-depende; calcium/calmodulin-dependent protein kinase II alpha

other names :

calcium/calmodulin-dependent protein kinase type II subunit alpha; Calcium/calmodulin-dependent protein kinase type II subunit alpha; calcium/calmodulin-dependent protein kinase type II subunit alpha; alpha CaM kinase II; caMK-II subunit alpha; caM-kinase II alpha chain; caM kinase II subunit alpha; Ca2+/calmodulin-dependent protein kinase II alpha; calcium/calmodulin-dependent protein kinase II alpha subunit; calcium/calmodulin-dependent protein kinase type II alpha chain; calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha; calcium/calmodulin-dependent protein kinase II alpha

products gene name :

CAMK2A

other gene names :

Camk2a; Camk2a; PKCCD; PK2CDD; CaM kinase II subunit alpha; CaMK-II subunit alpha

uniprot entry name :

KCC2A_RAT

reactivity :

Rat

sequence length :

478

specificity :

This assay has high sensitivity and excellent specificity for detection of rat CAMK 2. No significant cross-reactivity or interference between rat CAMK 2 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml -1000 pg/ml. Sensitivity: The minimum detectable dose of rat CAMK 2 is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CAMK 2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CAMK 2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CAMK 2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CAMK 2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

6978593

ncbi acc num :

NP_037052.1

ncbi gb acc num :

NM_012920.1

uniprot acc num :

P11275

ncbi mol weight :

54,115 Da

ncbi pathways :

Adrenergic Signaling In Cardiomyocytes Pathway (908278); Adrenergic Signaling In Cardiomyocytes Pathway (909696); Amphetamine Addiction Pathway (547642); Amphetamine Addiction Pathway (550546); Calcium Regulation In The Cardiac Cell Pathway (198495); Calcium Signaling Pathway (83442); Calcium Signaling Pathway (459); Cholinergic Synapse Pathway (217713); Circadian Entrainment Pathway (698787); Circadian Entrainment Pathway (699872)

uniprot summary :

Function: CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity.1 PublicationManual assertion based on experiment in:Ref.8. Catalytic activity: ATP + a protein = ADP + a phosphoprotein. Enzyme regulation: Autophosphorylation of Thr-286 allows the kinase to switch from a calmodulin-dependent to a calmodulin-independent state.1 PublicationManual assertion based on experiment in:Ref.6

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

810

size3 :

5x96-Strip-Wells

price3 :

2750

size4 :

10x96-Strip-Wells

price4 :

5150

more info or order :

MyBioSource product webpage