catalog number :
MBS702706
products type :
ELISA Kit
products full name :
Mouse Dentin Matrix Protein 1, DMP1 ELISA Kit
products short name :
dentin matrix acidic phosphoprotein 1
products name syn :
Mouse Dentin Matrix Protein 1 (DMP1) ELISA Kit; ARHP; ARHR; DMP-1; dentin matrix protein 1; dentin matrix acidic phosphoprotein 1
other names :
dentin matrix acidic phosphoprotein 1; Dentin matrix acidic phosphoprotein 1; dentin matrix acidic phosphoprotein 1; AG1; DMP-1; serine rich acidic phosphoprotein; dentin matrix protein 1; AG1
products gene name :
DMP1
other gene names :
Dmp1; Dmp1; PP; Dmp; AV020965; Dmp; DMP-1; Dentin matrix protein 1
uniprot entry name :
DMP1_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse DMP1. No significant cross-reactivity or interference between mouse DMP1 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 1.56 pg/ml -100 pg/ml. Sensitivity: The minimum detectable dose of mouse DMP1 is typically less than 0.39 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for DMP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DMP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DMP1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DMP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_058059.2
ncbi gb acc num :
NM_016779.2
ncbi mol weight :
53,965 Da
ncbi pathways :
ECM Proteoglycans Pathway (1001027); Extracellular Matrix Organization Pathway (1001015)
uniprot summary :
Function: May have a dual function during osteoblast differentiation. In the nucleus of undifferentiated osteoblasts, unphosphorylated form acts as a transcriptional component for activation of osteoblast-specific genes like osteocalcin. During the osteoblast to osteocyte transition phase it is phosphorylated and exported into the extracellular matrix, where it regulates nucleation of hydroxyapatite
size4 :
10x96-Strip-Wells