Human soluble CD163, sCD163 ELISA Kit | MyBioSource MBS702685 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human soluble CD163, sCD163 ELISA Kit

catalog :

MBS702685

quantity :

48-Strip-Wells

price :

505 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS702685

products type :

ELISA Kit

products full name :

Human soluble CD163, sCD163 ELISA Kit

products short name :

CD163 molecule

products name syn :

Human soluble CD163 (sCD163) ELISA Kit; M130; MM130; CD163 antigen; macrophage-associated antigen; CD163 molecule

other names :

scavenger receptor cysteine-rich type 1 protein M130 isoform a; Scavenger receptor cysteine-rich type 1 protein M130; scavenger receptor cysteine-rich type 1 protein M130; hemoglobin scavenger receptor; macrophage-associated antigen; CD163 molecule; Hemoglobin scavenger receptor; CD_antigen: CD163Cleaved into the following chain:Soluble CD163; sCD163

products gene name :

CD163

other gene names :

CD163; CD163; M130; MM130; M130; sCD163

uniprot entry name :

C163A_HUMAN

reactivity :

Human

sequence length :

1156

specificity :

This assay has high sensitivity and excellent specificity for detection of human sCD163. No significant cross-reactivity or interference between human sCD163 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Quantitative Sandwich. Sensitivity: Typically less than 0.39 ng/ml.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for sCD163 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any sCD163 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for sCD163 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of sCD163 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

344179110

ncbi acc num :

NP_004235.4

ncbi gb acc num :

NM_004244.5

uniprot acc num :

Q86VB7

ncbi mol weight :

125,451 Da

ncbi pathways :

Binding And Uptake Of Ligands By Scavenger Receptors Pathway (771599); Scavenging Of Heme From Plasma Pathway (771600)

ncbi summary :

The protein encoded by this gene is a member of the scavenger receptor cysteine-rich (SRCR) superfamily, and is exclusively expressed in monocytes and macrophages. It functions as an acute phase-regulated receptor involved in the clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages, and may thereby protect tissues from free hemoglobin-mediated oxidative damage. This protein may also function as an innate immune sensor for bacteria and inducer of local inflammation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Aug 2011]

uniprot summary :

Function: Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells.5 PublicationsManual assertion based on experiment in:Ref.7

size1 :

48-Strip-Wells

price1 :

505 USD

size2 :

96-Strip-Wells

price2 :

715

size3 :

5x96-Strip-Wells

price3 :

2530

size4 :

10x96-Strip-Wells

price4 :

4730

more info or order :

MyBioSource product webpage