catalog number :
MBS702671
products type :
ELISA Kit
products full name :
Mouse Phospholipidase A2, PL-A2 ELISA Kit
products short name :
phospholipase A2, group IB (pancreas)
products name syn :
Mouse Phospholipidase A2; PL-A2 ELISA Kit; MGC119834; MGC119835; PLA2; PLA2A; PPLA2; phosphatidylcholine 2-acylhydrolase; phospholipase A2 group IB; phospholipase A2; group IB (pancreas)
other names :
phospholipase A2; Phospholipase A2; phospholipase A2; PLA2-Ib; group IB phospholipase A2; phosphatidylcholine 2-acylhydrolase 1B; phospholipase A2, group IB, pancreas; Group IB phospholipase A2; PLA2-Ib; Phosphatidylcholine 2-acylhydrolase 1B
products gene name :
PLA2G1B
other gene names :
Pla2g1b; Pla2g1b; Pla2a; sPLA2IB; Pla2
uniprot entry name :
PA21B_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of Mouse PLA2G1B. No significant cross-reactivity or interference between Mouse PLA2G1B and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, cell culture supernates, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 1.25 ng/ml-80 ng/ml. Sensitivity: 0.31 ng/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Biosynthesis/Metabolism. Protein Biological Process 2: Lipogenesis and lipometabolism. Protein Biological Process 3: Lipid degradation
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PL-A2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PL-A2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PL-A2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PL-A2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_035237.1
ncbi gb acc num :
NM_011107.1
ncbi mol weight :
16,290 Da
ncbi pathways :
Acyl Chain Remodelling Of PC Pathway (1001074); Acyl Chain Remodelling Of PE Pathway (1001070); Acyl Chain Remodelling Of PG Pathway (1001081); Acyl Chain Remodelling Of PI Pathway (1001079); Acyl Chain Remodelling Of PS Pathway (1001077); Arachidonic Acid Metabolism Pathway (83190); Arachidonic Acid Metabolism Pathway (366); Ether Lipid Metabolism Pathway (83189); Ether Lipid Metabolism Pathway (365); Fat Digestion And Absorption Pathway (194386)
uniprot summary :
Function: PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides, this releases glycerophospholipids and arachidonic acid that serve as the precursors of signal molecules. Catalytic activity: Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate. Cofactor: Binds 1 calcium ion per subunit
size4 :
10x96-Strip-Wells