ELISA/assay

Human beta-actin ELISA Kit

MBS702655

48-Strip-Wells

510 USD

ELISA Kit

Human beta-actin ELISA Kit

actin, beta

Human beta-actin ELISA Kit; PS1TP5BP1; PS1TP5-binding protein 1; actin; cytoplasmic 1; beta actin; beta cytoskeletal actin; actin; beta

actin, cytoplasmic 1; Actin, cytoplasmic 1; actin, cytoplasmic 1; beta cytoskeletal actin; PS1TP5-binding protein 1; actin, beta; Beta-actinCleaved into the following chain:Actin, cytoplasmic 1, N-terminally processed

ACTB

ACTB; ACTB; BRWS1; PS1TP5BP1

ACTB_HUMAN

Human

375

This assay has high sensitivity and excellent specificity for detection of human beta-actin. No significant cross-reactivity or interference between human beta-actin and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates, cell lysates. Assay Type: Sandwich. Detection Range: 0.312 ng/ml -20 ng/ml. Sensitivity: 0.078 ng/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for beta-actin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any beta-actin present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for beta-actin is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of beta-actin bound in the initial step. The color development is stopped and the intensity of the color is measured.

4501885

NP_001092.1

NM_001101.3

P60709

41,737 Da

Adherens Junction Pathway (83070); Adherens Junction Pathway (481); Arrhythmogenic Right Ventricular Cardiomyopathy Pathway (672454); Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway (117293); Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway (116129); Axon Guidance Pathway (105688); Bacterial Invasion Of Epithelial Cells Pathway (149807); Bacterial Invasion Of Epithelial Cells Pathway (148661); Chaperonin-mediated Protein Folding Pathway (106242); Chromatin Modifying Enzymes Pathway (941403)

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008]

Function: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800