Human Protein DJ-1, PARK7 ELISA Kit | MyBioSource MBS702647 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Protein DJ-1, PARK7 ELISA Kit

catalog :

MBS702647

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS702647

products type :

ELISA Kit

products full name :

Human Protein DJ-1, PARK7 ELISA Kit

products short name :

Parkinson disease (autosomal recessive, early onset) 7

products name syn :

Human Protein DJ-1 (PARK7) ELISA kit; CTA-215D11.1; DJ-1; DJ1; FLJ27376; FLJ34360; FLJ92274; OTTHUMP00000001350; OTTHUMP00000001351; Parkinson disease protein 7; oncogene DJ1; protein DJ-1; Parkinson disease (autosomal recessive; early onset) 7

other names :

protein DJ-1; Protein DJ-1; protein DJ-1; oncogene DJ1; epididymis secretory sperm binding protein Li 67p; Parkinson disease (autosomal recessive, early onset) 7; parkinson protein 7; Oncogene DJ1; Parkinson disease protein 7

products gene name :

PARK7

other gene names :

PARK7; PARK7; DJ1; DJ-1; HEL-S-67p

uniprot entry name :

PARK7_HUMAN

reactivity :

Human

sequence length :

189

specificity :

This assay has high sensitivity and excellent specificity for detection of human PARK7. No significant cross-reactivity or interference between human PARK7 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, tissue homogenates and Cell lysates. Assay Type: Sandwich. Detection Range: 0.235 ng/ml -15 ng/ml. Sensitivity: The minimum detectable dose of human PARK7 is typically less than 0.058 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

. Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PARK7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PARK7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PARK7 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PARK7 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

183227678

ncbi acc num :

NP_001116849.1

ncbi gb acc num :

NM_001123377.1

uniprot acc num :

Q99497

ncbi mol weight :

19,891 Da

ncbi pathways :

Alpha-synuclein Signaling Pathway (137913); Androgen Receptor Signaling Pathway (198806); Parkinson's Disease Pathway (83098); Parkinsons Disease Pathway (705377); Synaptic Vesicle Pathway (672457)

ncbi summary :

The product of this gene belongs to the peptidase C56 family of proteins. It acts as a positive regulator of androgen receptor-dependent transcription. It may also function as a redox-sensitive chaperone, as a sensor for oxidative stress, and it apparently protects neurons against oxidative stress and cell death. Defects in this gene are the cause of autosomal recessive early-onset Parkinson disease 7. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

Function: Protects cells against oxidative stress and cell death. Plays a role in regulating expression or stability of the mitochondrial uncoupling proteins SLC25A14 and SLC25A27 in dopaminergic neurons of the substantia nigra pars compacta and attenuates the oxidative stress induced by calcium entry into the neurons via L-type channels during pacemaking. Eliminates hydrogen peroxide and protects cells against hydrogen peroxide-induced cell death. May act as an atypical peroxiredoxin-like peroxidase that scavenges hydrogen peroxide. Following removal of a C-terminal peptide, displays protease activity and enhanced cytoprotective action against oxidative stress-induced apoptosis. Stabilizes NFE2L2 by preventing its association with KEAP1 and its subsequent ubiquitination. Binds to OTUD7B and inhibits its deubiquitinating activity. Enhances RELA nuclear translocation. Binds to a number of mRNAs containing multiple copies of GG or CC motifs and partially inhibits their translation but dissociates following oxidative stress. Required for correct mitochondrial morphology and function and for autophagy of dysfunctional mitochondria. Regulates astrocyte inflammatory responses. Acts as a positive regulator of androgen receptor-dependent transcription. Prevents aggregation of SNCA. Plays a role in fertilization. Has no proteolytic activity. Has cell-growth promoting activity and transforming activity. May function as a redox-sensitive chaperone. May regulate lipid rafts-dependent endocytosis in astrocytes and neuronal cells.15 PublicationsManual assertion based on experiment in:Ref.1

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

810

size3 :

5x96-Strip-Wells

price3 :

2750

size4 :

10x96-Strip-Wells

price4 :

5150

more info or order :

MyBioSource product webpage