ELISA/assay

Rabbit Myeloperoxidase, MPO ELISA Kit

MBS702642

48-Strip-Wells

565 USD

ELISA Kit

Rabbit Myeloperoxidase, MPO ELISA Kit

Myeloperoxidase, MPO

Rabbit Myeloperoxidase (MPO) ELISA kit; Myeloperoxidase; MPO

myeloperoxidase; Myeloperoxidase; myeloperoxidase; myeloperoxidase

MPO

MPO; MPO; MPO

PERM_HUMAN

Rabbit

745

This assay has high sensitivity and excellent specificity for detection of rabbit MPO. No significant cross-reactivity or interference between rabbit MPO and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Competitive. Detection Range: 1.25 ng/ml -20 ng/ml. Sensitivity: 1.25 ng/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to MPO. Standards or samples are added to the appropriate microtiter plate wells with Biotin-conjugated MPO. A competitive inhibition reaction is launched between MPO (Standards or samples) and Biotin-conjugated MPO with the pre-coated antibody specific for MPO. The more amount of MPO in samples, the less antibody bound by Biotin-conjugated MPO. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of MPO in the sample. The color development is stopped and the intensity of the color is measured.

4557759

NP_000241.1

NM_000250.1

P05164

87,248 Da

C-MYB Transcription Factor Network Pathway (138073); Folate Metabolism Pathway (198833); IL23-mediated Signaling Events Pathway (138000); Phagosome Pathway (153910); Phagosome Pathway (153859); Selenium Pathway (198825); Transcriptional Misregulation In Cancer Pathway (523016); Transcriptional Misregulation In Cancer Pathway (522987); Vitamin B12 Metabolism Pathway (920974); Amb2 Integrin Signaling Pathway (137945)

Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor, myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of netrophils. [provided by RefSeq, Jul 2008]

Function: Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. Catalytic activity: Cl- + H2O2 + H+ = HClO + H2O. Cofactor: Binds 1 calcium ion per monomer.

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150