ELISA/assay

Rat heme oxygenase 1, HO-1 ELISA Kit

MBS702641

96 Strip Wells

915 USD

ELISA Kit

Rat heme oxygenase 1, HO-1 ELISA Kit

heme oxygenase (decycling) 1

Rat heme oxygenase 1; HO-1 ELISA Kit; HO-1; HSP32; bK286B10; heme oxygenase (decyclizing) 1; heme oxygenase (decycling) 1

heme oxygenase 1; Heme oxygenase 1; heme oxygenase 1; heme oxygenase (decyclizing) 1; heme oxygenase (decycling) 1; HSP32

HMOX1

Hmox1; Hmox1; Ho1; Heox; Hmox; Ho-1; HEOXG; hsp32; HO-1

HMOX1_RAT

Rat

289

This assay has high sensitivity and excellent specificity for detection of Rat HMOX1. No significant cross-reactivity or interference between Rat HMOX1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, tissue homogenates. Detection Range: 0.312 ng/ml-20 ng/ml. Detection Wavelength: 450 nm. Sensitivity: 0.078 ng/ml

Sample Volume: 50-100ul

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HO-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HO-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HO-1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HO-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

6981032

NP_036712.1

NM_012580.2

P06762

33,006 Da

HIF-1 Signaling Pathway 695233!!Heme Degradation Pathway 1010848!!Iron Uptake And Transport Pathway 1010008!!Keap1-Nrf2 Pathway 219759!!Metabolism Pathway 1010675!!Metabolism Of Porphyrins Pathway 1010846!!Mineral Absorption Pathway 212234!!Mineral Absorption Pathway 212220!!NFE2L2 Pathway 739005!!Oxidative Stress Pathway 198512

catalyzes the oxidative cleavage of heme to biliverdin [RGD, Feb 2006]

Function: Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Exhibits cytoprotective effects since excess of free heme sensitizes cells to undergo apoptosis. Catalytic activity: Protoheme + 3 AH2 + 3 O2 = biliverdin + Fe2+ + CO + 3 A + 3 H2O.

96 Strip Wells

915 USD