ELISA/assay

Mouse Klotho ELISA Kit

MBS702609

48-Strip-Wells

510 USD

ELISA Kit

Mouse Klotho ELISA Kit

klotho

Mouse Klotho ELISA Kit; klotho

klotho; Klotho; klotho; secreted form of Klotho protein; klotho

KL

Kl; Kl; alpha-kl

KLOT_MOUSE

Mouse

1014

This assay has high sensitivity and excellent specificity for detection of mouse klotho. No significant cross-reactivity or interference between mouse klotho and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, cell culture supernates. Assay Type: Sandwich. Detection Range: 3.12 pg/ml -200 pg/ml. Sensitivity: 0.78 pg/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for klotho has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any klotho present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for klotho is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of klotho bound in the initial step. The color development is stopped and the intensity of the color is measured.

227330584

NP_038851.2

NM_013823.2

O35082

116,398 Da

Adaptive Immune System Pathway (971210); Ascorbate Biosynthesis, Animals, Glucose-1P = Ascorbate Pathway (421792); Ascorbate Biosynthesis, Animals, Glucose-1P = Ascorbate Pathway (468309); Constitutive PI3K/AKT Signaling In Cancer Pathway (1000676); DAP12 Interactions Pathway (971203); DAP12 Signaling Pathway (971214); Disease Pathway (970606); Downstream Signal Transduction Pathway (971469); Downstream Signaling Events Of B Cell Receptor (BCR) Pathway (1001193); Downstream Signaling Of Activated FGFR Pathway (971322)

Function: May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 241 and 874, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D. Essential factor for the specific interaction between FGF23 and FGFR1.6 PublicationsManual assertion based on experiment in:Ref.1. Catalytic activity: A beta-D-glucuronoside + H2O = D-glucuronate + an alcohol.2 PublicationsManual assertion based on experiment in:Ref.13. Enzyme regulation: Inhibited by D-saccharic acid 1,4-lactone and taurocholic acid.1 PublicationManual assertion based on experiment in:Ref.13

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800