Human clusterin, CLU ELISA Kit | MyBioSource MBS702592 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human clusterin, CLU ELISA Kit

catalog :

MBS702592

quantity :

24-Strip-Wells (LIMIT 1)

price :

240 USD

product information

catalog number :

MBS702592

products type :

ELISA Kit

products full name :

Human clusterin, CLU ELISA Kit

products short name :

[clusterin]

products name syn :

[Human clusterin; CLU ELISA Kit; AAG4; APOJ; CLI; KUB1; MGC24903; SGP-2; SGP2; SP-40; TRPM-2; TRPM2; aging-associated protein 4; apolipoprotein J; clusterin (complement lysis inhibitor; SP-40; 40; sulfated glycoprotein 2; testosterone; clusterin]

other names :

[clusterin preproprotein; Clusterin; clusterin; apolipoprotein J; ku70-binding protein 1; sulfated glycoprotein 2; aging-associated protein 4; complement lysis inhibitor; complement cytolysis inhibitor; complement-associated protein SP-40,40; testosterone-repressed prostate message 2; clusterin; Aging-associated gene 4 protein; Apolipoprotein J; Apo-J; Complement cytolysis inhibitor; CLI; Complement-associated protein SP-40,40; Ku70-binding protein 1; NA1/NA2; Testosterone-repressed prostate message 2; TRPM-2]

products gene name :

[CLU]

other gene names :

[CLU; CLU; CLI; AAG4; APOJ; CLU1; CLU2; KUB1; SGP2; APO-J; SGP-2; SP-40; TRPM2; TRPM-2; NA1/NA2; APOJ; CLI; KUB1; AAG4; Apo-J; CLI; TRPM-2]

uniprot entry name :

CLUS_HUMAN

reactivity :

Human

sequence length :

449

specificity :

This assay has high sensitivity and excellent specificity for detection of Human CLU. No significant cross-reactivity or interference between Human CLU and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, plasma, cell culture supernates, saliva, urine. Assay Type: Sandwich. Detection Range: 3.12 ng/ml-200 ng/ml. Sensitivity: 0.78 ng/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Apoptosis/Autophagy. Protein Biological Process 3: Apoptosis

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CLU has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CLU present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CLU is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CLU bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

355594753

ncbi acc num :

NP_001822.3

ncbi gb acc num :

NM_001831.3

uniprot acc num :

P10909

ncbi mol weight :

52,495 Da

ncbi pathways :

Hemostasis Pathway (106028); Platelet Activation, Signaling And Aggregation Pathway (106034); Platelet Degranulation Pathway (106050); Response To Elevated Platelet Cytosolic Ca2+ Pathway (106048); Validated Targets Of C-MYC Transcriptional Repression Pathway (169353)

ncbi summary :

The protein encoded by this gene is a secreted chaperone that can under some stress conditions also be found in the cell cytosol. It has been suggested to be involved in several basic biological events such as cell death, tumor progression, and neurodegenerative disorders. Alternate splicing results in both coding and non-coding variants.[provided by RefSeq, May 2011]

uniprot summary :

Function: Isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. Secreted isoform 1 protects cells against apoptosis and against cytolysis by complement. Intracellular isoforms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis. Mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Plays a role in the regulation of cell proliferation.15 PublicationsManual assertion based on experiment in:Ref.24

size1 :

24-Strip-Wells (LIMIT 1)

price1 :

240 USD

size2 :

48-Strip-Wells

price2 :

550

size3 :

96-Strip-Wells

price3 :

795

size4 :

5x96-Strip-Wells

price4 :

2890

size5 :

10x96-Strip-Wells

price5 :

5465