Mouse hypoxia-inducible factor 1alpha, HIF-1alpha ELISA Kit | MyBioSource MBS702590 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse hypoxia-inducible factor 1alpha, HIF-1alpha ELISA Kit

catalog :

MBS702590

quantity :

48-Strip-Wells

price :

590 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS702590

products type :

ELISA Kit

products full name :

Mouse hypoxia-inducible factor 1alpha, HIF-1alpha ELISA Kit

products short name :

hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)

products name syn :

Mouse hypoxia-inducible factor 1alpha; HIF-1alpha ELISA Kit; HIF-1alpha; HIF1; HIF1-ALPHA; MOP1; PASD8; bHLHe78; ARNT interacting protein; hypoxia-inducible factor 1 alpha isoform I.3; hypoxia-inducible factor 1; alpha subunit; hypoxia-inducible factor 1; alpha; hypoxia inducible factor 1; alpha subunit (basic helix-loop-helix transcription factor)

other names :

hypoxia-inducible factor 1-alpha; Hypoxia-inducible factor 1-alpha; hypoxia-inducible factor 1-alpha; HIF1-alpha; HIF-1-alpha; ARNT-interacting protein; hypoxia inducible factor 1, alpha subunit; ARNT-interacting protein

products gene name :

HIF1A

other gene names :

Hif1a; Hif1a; MOP1; bHLHe78; AA959795; HIF1alpha; HIF-1-alpha; HIF1-alpha

uniprot entry name :

HIF1A_MOUSE

reactivity :

Mouse

sequence length :

836

specificity :

This assay has high sensitivity and excellent specificity for detection of mouse HIF-1alpha. No significant cross-reactivity or interference between mouse HIF-1alpha and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma. Assay Type: Sandwich. Detection Range: 0.625 pg/ml -40 pg/ml. Sensitivity: 0.156 pg/ml.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HIF-1alpha has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HIF-1alpha present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HIF-1alpha is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HIF-1alpha bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

226061948

ncbi acc num :

NP_034561.2

ncbi gb acc num :

NM_010431.2

uniprot acc num :

Q61221

ncbi mol weight :

93,516 Da

ncbi pathways :

Adipogenesis Pathway (198299); Cellular Response To Hypoxia Pathway (1000311); Cellular Responses To Stress Pathway (971022); Circadian Clock Pathway (1000461); Disease Pathway (970606); FBXW7 Mutants And NOTCH1 In Cancer Pathway (1000689); HIF-1 Signaling Pathway (695223); NOTCH1 Intracellular Domain Regulates Transcription Pathway (1000682); Oxygen-dependent Asparagine Hydroxylation Of Hypoxia-inducible Factor Alpha Pathway (1000313); Oxygen-dependent Proline Hydroxylation Of Hypoxia-inducible Factor Alpha Pathway (1000314)

ncbi summary :

This gene encodes the alpha subunit which, along with the beta subunit, forms a heterodimeric transcription factor that regulates the cellular and developmental response to reduced oxygen tension. The transcription factor has been shown to regulate genes involved in several biological processes, including erythropoiesis and angiogenesis which aid in increased delivery of oxygen to hypoxic regions. The transcription factor also plays a role in the induction of genes involved in cell proliferation and survival, energy metabolism, apoptosis, and glucose and iron metabolism. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]

uniprot summary :

Function: Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia 2 PublicationsManual assertion based on experiment in:Ref.9

size1 :

48-Strip-Wells

price1 :

590 USD

size2 :

96-Strip-Wells

price2 :

850

size3 :

5x96-Strip-Wells

price3 :

2880

size4 :

10x96-Strip-Wells

price4 :

5440

more info or order :

MyBioSource product webpage