ELISA/assay

Human alpha-L-fucosidase, AFU ELISA Kit

MBS702582

48-Strip-Wells

565 USD

ELISA Kit

Human alpha-L-fucosidase, AFU ELISA Kit

fucosidase, alpha-L- 2, plasma

Human alpha-L-fucosidase; AFU ELISA Kit; RP1-20N2.5; MGC1314; dJ20N2.5; ; fucosidase; alpha-L- 2; plasma

plasma alpha-L-fucosidase; Plasma alpha-L-fucosidase; plasma alpha-L-fucosidase; alpha-L-fucosidase 2; alpha-L-fucoside fucohydrolase 2; fucosidase, alpha-L- 2, plasma; Alpha-L-fucoside fucohydrolase 2; Alpha-L-fucosidase 2

FUCA2

FUCA2; FUCA2; dJ20N2.5; PSEC0151; UNQ227/PRO260; Alpha-L-fucosidase 2

FUCO2_HUMAN

Human

467

This assay has high sensitivity and excellent specificity for detection of human AFU. No significant cross-reactivity or interference between human AFU and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 31.25 uU/ml -2000 uU/ml. Sensitivity: The minimum detectable dose of human AFU is typically less than 7.8 uU/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for AFU has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AFU present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AFU is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AFU bound in the initial step. The color development is stopped and the intensity of the color is measured.

40068512

NP_114409.2

NM_032020.4

Q9BTY2

54,067 Da

Other Glycan Degradation Pathway (82976); Other Glycan Degradation Pathway (346)

This gene encodes a plasma alpha-L-fucosidase, which represents 10-20% of the total cellular fucosidase activity. The protein is a member of the glycosyl hydrolase 29 family, and catalyzes the hydrolysis of the alpha-1,6-linked fucose joined to the reducing-end N-acetylglucosamine of the carbohydrate moieties of glycoproteins. This enzyme is essential for Helicobacter pylori adhesion to human gastric cancer cells. [provided by RefSeq, Aug 2010]

Function: Alpha-L-fucosidase is responsible for hydrolyzing the alpha-1,6-linked fucose joined to the reducing-end N-acetylglucosamine of the carbohydrate moieties of glycoproteins. Catalytic activity: An alpha-L-fucoside + H2O = L-fucose + an alcohol.

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150