Human A Disintegrin And Metalloprotease 15, ADAM15 ELISA Kit | MyBioSource MBS702567 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human A Disintegrin And Metalloprotease 15, ADAM15 ELISA Kit

catalog :

MBS702567

quantity :

48-Strip-Wells

price :

505 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS702567

products type :

ELISA Kit

products full name :

Human A Disintegrin And Metalloprotease 15, ADAM15 ELISA Kit

products short name :

ADAM metallopeptidase domain 15

products name syn :

Human A Disintegrin And Metalloprotease 15; ADAM15 ELISA Kit; RP11-540D14.1; MDC15; a disintegrin and metalloproteinase domain 15; a disintegrin and metalloproteinase domain 15 (metargidin) ; metalloprotease RGD disintegrin protein; metalloproteinase-like; disinte; ADAM metallopeptidase domain 15

other names :

disintegrin and metalloproteinase domain-containing protein 15 isoform 7 preproprotein; Disintegrin and metalloproteinase domain-containing protein 15; disintegrin and metalloproteinase domain-containing protein 15; MDC-15; metalloprotease RGD disintegrin protein; a disintegrin and metalloproteinase domain 15 (metargidin); metalloproteinase-like, disintegrin-like, and cysteine-rich protein 15; ADAM metallopeptidase domain 15; Metalloprotease RGD disintegrin protein; Metalloproteinase-like, disintegrin-like, and cysteine-rich protein 15; MDC-15; Metargidin

products gene name :

ADAM15

other gene names :

ADAM15; ADAM15; MDC15; MDC15; ADAM 15; MDC-15

uniprot entry name :

ADA15_HUMAN

reactivity :

Human

sequence length :

824

specificity :

This assay has high sensitivity and excellent specificity for detection of Human ADAM15. No significant cross-reactivity or interference between Human ADAM15 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.625 ng/ml-40 ng/ml. Sensitivity: 0.156 ng/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Angiogenesis. Protein Biological Process 3: Angiogenesis

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ADAM15 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADAM15 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADAM15 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADAM15 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

387849014

ncbi acc num :

NP_001248393.1

ncbi gb acc num :

NM_001261464.1

uniprot acc num :

Q13444

ncbi mol weight :

92,959 Da

ncbi pathways :

Degradation Of The Extracellular Matrix Pathway (576263); Extracellular Matrix Organization Pathway (576262)

ncbi summary :

The protein encoded by this gene is a member of the ADAM (a disintegrin and metalloproteinase) protein family. ADAM family members are type I transmembrane glycoproteins known to be involved in cell adhesion and proteolytic ectodomain processing of cytokines and adhesion molecules. This protein contains multiple functional domains including a zinc-binding metalloprotease domain, a disintegrin-like domain, as well as a EGF-like domain. Through its disintegrin-like domain, this protein specifically interacts with the integrin beta chain, beta 3. It also interacts with Src family protein-tyrosine kinases in a phosphorylation-dependent manner, suggesting that this protein may function in cell-cell adhesion as well as in cellular signaling. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

uniprot summary :

Function: Active metalloproteinase with gelatinolytic and collagenolytic activity. Plays a role in the wound healing process. Mediates both heterotypic intraepithelial cell/T-cell interactions and homotypic T-cell aggregation. Inhibits beta-1 integrin-mediated cell adhesion and migration of airway smooth muscle cells. Suppresses cell motility on or towards fibronectin possibly by driving alpha-v/beta-1 integrin (ITAGV-ITGB1) cell surface expression via ERK1/2 inactivation. Cleaves E-cadherin in response to growth factor deprivation. Plays a role in glomerular cell migration. Plays a role in pathological neovascularization. May play a role in cartilage remodeling. May be proteolytically processed, during sperm epididymal maturation and the acrosome reaction. May play a role in sperm-egg binding through its disintegrin domain.7 PublicationsManual assertion based on experiment in:Ref.3. Cofactor: Binds 1 zinc ion per subunit . Enzyme regulation: Inhibited by hydroxamate-type metalloproteinase inhibitors such as marimastat. Inhibited by metalloproteinase inhibitor 2 (TIMP-2) and TIMP-3 at nanomolar concentrations. Not significantly inhibited by TIMP-1 at concentrations of up to 100 nM. Not activated by PMA or ionomycin.1 PublicationManual assertion based on experiment in:Ref.25

size1 :

48-Strip-Wells

price1 :

505 USD

size2 :

96-Strip-Wells

price2 :

715

size3 :

5x96-Strip-Wells

price3 :

2530

size4 :

10x96-Strip-Wells

price4 :

4730

more info or order :

MyBioSource product webpage