catalog number :
MBS702560
products type :
ELISA Kit
products full name :
Rat Synaptophysin, Syp ELISA Kit
products short name :
synaptophysin
products name syn :
Rat Synaptophysin (Syp) ELISA kit; MRXSYP; major synaptic vesicle protein P38; synaptophysin
other names :
synaptophysin; Synaptophysin; synaptophysin; major synaptic vesicle protein p38; synaptophysin; Major synaptic vesicle protein p38
other gene names :
Syp; Syp; Syp1
uniprot entry name :
SYPH_RAT
specificity :
This assay has high sensitivity and excellent specificity for detection of Rat SYP. No significant cross-reactivity or interference between Rat SYP and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Tissue homogenates, serum and plasma. Assay Type: Sandwich. Detection Range: 0.625 ng/ml-40 ng/ml. Sensitivity: 0.156 ng/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for synaptophysin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any synaptophysin present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for synaptophysin is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of synaptophysin bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_036796.1
ncbi gb acc num :
NM_012664.2
ncbi mol weight :
33,311 Da
ncbi summary :
may play a role in synaptic vesicle organization and synaptic plasticity [RGD, Feb 2006]
uniprot summary :
Function: Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity
size4 :
10x96-Strip-Wells