catalog number :
MBS702553
products type :
ELISA Kit
products full name :
Sheep Prolactin/Luteotropic Hormone, PRL/LTH ELISA Kit
products short name :
prolactin
products name syn :
Sheep Prolactin/Luteotropic Hormone (PRL/LTH) ELISA Kit; prolactin
other names :
prolactin; Prolactin; prolactin
other gene names :
PRL; PRL; PRL
uniprot entry name :
PRL_SHEEP
specificity :
This assay has high sensitivity and excellent specificity for detection of sheep PRL. No significant cross-reactivity or interference between sheep PRL and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Competitive. Detection Range: 5 muIU/ml - 2000 muIU/ml. Sensitivity: The minimum detectable dose of sheep PRL is typically less than 5 muIU/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest sheep PRL concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for PRL and Horseradish Peroxidase (HRP) conjugated PRL. The competitive inhibition reaction is launched between with HRP labeled PRL and unlabeled PRL with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of PRL in the sample. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001009306.1
ncbi gb acc num :
NM_001009306.1
ncbi mol weight :
25,778 Da
ncbi pathways :
Amyloids Pathway (366238); Cytokine Signaling In Immune System Pathway (366171); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Disease Pathway (530764); ErbB4 Signaling Events Pathway (137991); Glucocorticoid Receptor Regulatory Network Pathway (138014); Growth Hormone Receptor Signaling Pathway (477128); Immune System Pathway (106386); Jak-STAT Signaling Pathway (83077)
uniprot summary :
Function: Prolactin acts primarily on the mammary gland by promoting lactation, mammogenesis, mitogenesis and osmoregulation.
size4 :
10x96-Strip-Wells