Human glucocorticoid receptor-beta, GR-beta ELISA Kit | MyBioSource MBS702548 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human glucocorticoid receptor-beta, GR-beta ELISA Kit

catalog :

MBS702548

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS702548

products type :

ELISA Kit

products full name :

Human glucocorticoid receptor-beta, GR-beta ELISA Kit

products short name :

glucocorticoid receptor-beta, GR-beta

products name syn :

Human glucocorticoid receptor-beta; GR-beta ELISA Kit; glucocorticoid receptor-beta; GR-beta

other names :

glucocorticoid receptor isoform beta; Glucocorticoid receptor; glucocorticoid receptor; glucocorticoid nuclear receptor variant 1; nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor); Nuclear receptor subfamily 3 group C member 1

products gene name :

GR-beta

other gene names :

NR3C1; NR3C1; GR; GCR; GRL; GCCR; GRL; GR

uniprot entry name :

GCR_HUMAN

reactivity :

Human

sequence length :

742

specificity :

This assay has high sensitivity and excellent specificity for detection of human GR-beta. No significant cross-reactivity or interference between human GR-beta and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates and cell lysates. Assay Type: Sandwich. Detection Range: 39 pg/ml -2500 pg/ml. Sensitivity: 9.75 pg/ml.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GR-beta has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GR-beta present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GR-beta is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GR-beta bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

66528642

ncbi acc num :

NP_001018661.1

ncbi gb acc num :

NM_001020825.1

uniprot acc num :

P04150

ncbi mol weight :

82,904 Da

ncbi pathways :

Adipogenesis Pathway (198832); BMAL1:CLOCK,NPAS2 Activates Circadian Gene Expression Pathway (477138); Circadian Clock Pathway (187173); FOXA2 And FOXA3 Transcription Factor Networks Pathway (137911); Gene Expression Pathway (105937); Generic Transcription Pathway (105938); Glucocorticoid Receptor Regulatory Network Pathway (138014); Neuroactive Ligand-receptor Interaction Pathway (83053); Neuroactive Ligand-receptor Interaction Pathway (462); Nuclear Receptor Transcription Pathway (105979)

ncbi summary :

This gene encodes glucocorticoid receptor, which can function both as a transcription factor that binds to glucocorticoid response elements in the promoters of glucocorticoid responsive genes to activate their transcription, and as a regulator of other transcription factors. This receptor is typically found in the cytoplasm, but upon ligand binding, is transported into the nucleus. It is involved in inflammatory responses, cellular proliferation, and differentiation in target tissues. Mutations in this gene are associated with generalized glucocorticoid resistance. Alternative splicing of this gene results in transcript variants encoding either the same or different isoforms. Additional isoforms resulting from the use of alternate in-frame translation initiation sites have also been described, and shown to be functional, displaying diverse cytoplasm-to-nucleus trafficking patterns and distinct transcriptional activities (PMID:15866175). [provided by RefSeq, Feb 2011]

uniprot summary :

Function: Receptor for glucocorticoids (GC). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE), both for nuclear and mitochondrial DNA, and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth. Involved in chromatin remodeling. May play a negative role in adipogenesis through the regulation of lipolytic and antilipogenic genes expression.2 PublicationsManual assertion based on experiment in:Ref.37

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

810

size3 :

5x96-Strip-Wells

price3 :

2750

size4 :

10x96-Strip-Wells

price4 :

5150

more info or order :

MyBioSource product webpage