catalog number :
MBS702544
products type :
ELISA Kit
products full name :
Mouse coagulation factor VIII, FVIII ELISA Kit
products short name :
coagulation factor VIII, procoagulant component
products name syn :
Mouse coagulation factor VIII (FVIII) ELISA Kit; RP11-115M6.7; AHF; DXS1253E; F8B; F8C; FVIII; HEMA; coagulation factor VIII; coagulation factor VIIIc; factor VIII F8B; coagulation factor VIII; procoagulant component
other names :
coagulation factor VIII isoform 1; Coagulation factor VIII; coagulation factor VIII; Factor VIII; procoagulant component; coagulation factor VIII; Procoagulant component
other gene names :
F8; F8; Cf8; Cf-8; FVIII; Cf8; F8c
uniprot entry name :
FA8_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse FVIII. No significant cross-reactivity or interference between mouse FVIII and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma. Assay Type: Sandwich. Detection Range: 0.312 ng/ml-20 ng/ml. Sensitivity: 0.078 ng/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FVIII has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FVIII present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FVIII is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FVIII bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_032003.2
ncbi gb acc num :
NM_007977.2
ncbi mol weight :
266,196 Da
ncbi pathways :
Blood Clotting Cascade Pathway (198388); Complement And Coagulation Cascades Pathway (198335); Complement And Coagulation Cascades Pathway (83270); Complement And Coagulation Cascades Pathway (484); Formation Of Fibrin Clot (Clotting Cascade) Pathway (1001318); Hemostasis Pathway (1001295); Intrinsic Pathway (1001320); Platelet Activation, Signaling And Aggregation Pathway (1001305); Platelet Degranulation Pathway (1001317); Response To Elevated Platelet Cytosolic Ca2+ Pathway (1001315)
uniprot summary :
Function: Factor VIII, along with calcium and phospholipid, acts as a cofactor for factor IXa when it converts factor X to the activated form, factor Xa.
size4 :
10x96-Strip-Wells